Clinical Hemorheology and Microcirculation - Volume 6, issue 2

Article Type: Editorial

DOI: 10.3233/CH-1986-6217

Citation: Clinical Hemorheology and Microcirculation, vol. 6, no. 2, pp. I-III, 1986

Authors: Copley, A.L.

Article Type: Editorial

DOI: 10.3233/CH-1986-6201

Citation: Clinical Hemorheology and Microcirculation, vol. 6, no. 2, pp. 111-111, 1986

Authors: Di Perri, Tullio

Article Type: Other

DOI: 10.3233/CH-1986-6202

Citation: Clinical Hemorheology and Microcirculation, vol. 6, no. 2, pp. 113-116, 1986

Authors: Stuart, John

Article Type: Other

DOI: 10.3233/CH-1986-6203

Citation: Clinical Hemorheology and Microcirculation, vol. 6, no. 2, pp. 117-117, 1986

Authors: Ricci, Carlo

Article Type: Other

DOI: 10.3233/CH-1986-6204

Citation: Clinical Hemorheology and Microcirculation, vol. 6, no. 2, pp. 119-120, 1986

Authors: Grossi, Adalberto

Article Type: Other

DOI: 10.3233/CH-1986-6205

Citation: Clinical Hemorheology and Microcirculation, vol. 6, no. 2, pp. 121-122, 1986

Authors: Schmid-Schönbein, H.

Article Type: Research Article

Abstract: The two expert meetings on standardization of red cell filtrometry have uncovered significant ambiguities in our methodological and conceptional approaches to understand the normal and abnormal rheological characteristics of human blood. Severe methodological artefacts were identified. The present round table addresses these problems showing that presently popular whole blood filtration techniques are not only hampered by artefacts introduced by non-red cells (leukocytes of all subclasses and thrombocytes) but are strongly influenced by the mode of anticoagulation. Furthermore, in tests where these two variables are controlled, there is a very significant influence of small subpopulations of rigidified red cells on the …filtration characteristics on the entire red cell population. Conversely, there is increasing evidence suggesting that a very successful procedure suggested to remove leukocytes also removes the rigid subpopulations. Thence, the suspicion remains that cotton wool filtration introduces a new,albeit subtle artefact. Show more

DOI: 10.3233/CH-1986-6206

Citation: Clinical Hemorheology and Microcirculation, vol. 6, no. 2, pp. 123-125, 1986

Authors: Baar, S. | Bareford, D. | Keidan, A.J. | Stone, P.C.W. | Stuart, J.

Article Type: Research Article

Abstract: The Imugard IGSOO cotton wool pre-filtration technique, used to remove leucocytes from whole blood prior to study of erythrocyte deformability, has been tested to determine whether it selectively retains less deformable erythrocytes. Leucocyte-free suspensions of erythrocytes prepared in parallel by this technique and by centrifugation in narrow-bore Wintrobe macrohaematocrit tubes gave similar values for filtration through 5 µm pores in the Hemorheometre. Erythrocytes that passed through Imugard IG SOO cotton wool gave similar density profiles to whole blood when fractionated on a Percoll-Stractan continuous density gradient. Erythrocytes retained by, and subsequently eluted from, the cotton wool did not show impaired …deformability as measured by the Hemorheometre or Ektacytometer. We have been unable to demonstrate selective retention of less deformable erythrocytes by Imugard IGSOO cotton wool. Show more

Keywords: Rheology, Erythrocyte deformability, Leukocytes

DOI: 10.3233/CH-1986-6207

Citation: Clinical Hemorheology and Microcirculation, vol. 6, no. 2, pp. 127-136, 1986

Authors: Bartoli, V. | Albanese, B. | Manescalchi, P.G. | Mannini, L. | Pasquini, G.

Article Type: Research Article

Abstract: Experimental: Two anticoagulants were tested, K2-EDTA and lithium-heparin. Blood was collected in both glass and plastic tubes using EDTA and in glass tubes only when lithium-heparin was used in two forms: dry and in a dilute saline sample. EDTA anticoagulated blood samples collected in plastic tubes were stored at 4°C, room temperature and 37°C. Immediately after the venepuncture and 1, 2, 6 and 24 hours later the following tests were carried out: whole blood filtration (Reid et al., 1976) using Nuclepore Hemafil filters, blood and plasma viscosity at low and high shear rates (Contraves LS 30 and Brookfield LVT viscometers), …blood cell counts, red cell indices (Coulter counter S plus) and hematocrit. The morphology of erythrocytes was studied by means of Scanning Electron Microscopy (SEM) .The specimens of red cells were obtained from the same samples that underwent rheological tests. Results: Using heparin as an anticoagulant, whole blood filtration was severely impaired or blocked and blood viscosity and hematocrit were higher than they were using EDTA. The rheological properties of blood were altered by the storage, even after 1 hour, and the alteration became progressively greater as a function of time. SEM observations contributed to clarifying the effect of anticoagulants and storage conditions on the rheological properties of blood showing several changes of red blood cell morphology. Show more

Keywords: Anticoagulants, Blood storage, Blood filtration test

DOI: 10.3233/CH-1986-6208

Citation: Clinical Hemorheology and Microcirculation, vol. 6, no. 2, pp. 137-149, 1986

Authors: Seiffge, D. | Behr, S.

Article Type: Research Article

Abstract: The Single Erythrocyte Rigidometer (SER) has been applied to measure various extrinsic and intrinsic factors involved in red blood cell (RBC) passage time through a single pore membrane. The SER allows to measure the mean passage time (MPT in ms) of 250 single RBC’s each under a relevant and low constant pressure gradient of 70 Pa (τ = 3 Pa) through single pores of defined geometry (SEM measurements). Furthermore a computer program provides us with a list of every single RBC passage time, a flow distribution curve and the MPT of the 10, 5, 2.5, and 1% slowest RBC’s …(“subpopulation”). In the course of the present investigation extrinsic factors like pressure gradient and pore geometry have been examined. MPT correlates directly with pore diameter, RBC shape, and volume. Also the influence of leucocyte removal by cotton wool filtration has been examined. Even the filtration of control RBC increased the MPT by 9% (n = 47). Furthermore we evaluated the influence of different intrinsic factors on RBC deformability. To simulate the imbalanced metabolic situation under pathophysiological conditions we have changed in vitro different parameters. Buffers of different stress degree have been composed and applied to pharmacological characterisation of compounds. Additionally all RBC preparations have been checked for possible subpopulations of rigid cells. The detection of rigid subpopulations was dependent of the pore diameter and they were even detectable in control blood. Show more

Keywords: Single Erythrocyte Rigidometer, red cell deformability, stress factors, rigid subpopulations

DOI: 10.3233/CH-1986-6209

Citation: Clinical Hemorheology and Microcirculation, vol. 6, no. 2, pp. 151-164, 1986