Bio-Medical Materials and Engineering - Volume 24, issue 1

Authors: Yu, Deng-Guang | Li, Xiao-Yan | Chian, Wei | Li, Ying | Wang, Xia

Article Type: Research Article

Abstract: The influence of different types of solvents as sheath fluids on the quality of electrospun ethyl cellulose (EC) nanofibers is investigated in this paper by a modified coaxial process. With 24 w/v % EC in ethanol as electrospinning core fluid and pure solvents including methanol, ethanol and N,N-dimethyl formamide (DMF) as sheath fluids, EC nanofibers were generated by the modified processes. Field emission scanning electron microscope observations demonstrate that the modified process is effective in improving the nanofibers' quality in terms of nanofibers' diameters, distributions and structural uniformity. The key of the modified coaxial process is the reasonable selection of …the sheath solvents that is suitable for the drawing process of core EC fluid during the electrpospinning. The EC nanofibers' diameters (D, nm) could be manipulated through the reasonable selection of the type of the sheath solvents based on their boiling point (T, °C) D = 841–3.71T (R=0.9753). This paper provides useful methods for the implementation of the modified coaxial process controllably to obtain polymer nanofibers with high quality. Show more

Keywords: Coaxial electrospinning, sheath solvent, nanofibers, quality, ethyl cellulose

DOI: 10.3233/BME-130857

Citation: Bio-Medical Materials and Engineering, vol. 24, no. 1, pp. 695-701, 2014

Authors: Tang, Wenwei | Xia, Jin | Zeng, Xinping | Wu, Lujun | Ye, Guoqing

Article Type: Research Article

Abstract: A new manganese-oxidizing strain FM-2 was screened out from biological activated carbon (BAC) filter column and was identified as Citrobacter freundii. The results of the systematic study on this species are as follows: At 27°C, the optimum pH for Citrobacter sp. FM-2 to remove manganese was 7.0–8.0.The best removal rate of manganese under 27°C, pH 7.0 by FM-2 was reached at 4 d, being 76.2%; Compared with adsorption, biological oxidation played a dominant role in this removing process. Almost 75.7% of manganese was oxidized into oxides by Citrobacter sp and there were some particular oxides analogs generated on the bacterial …surface; A 296bp DNA fragment amplified from Citrobacter sp. FM-2 revealed that this species has multicopper oxidase genes. Meanwhile, the phylogenetic tree indicated that compared with other related species, Citrobacter sp. FM-2 has its own evolutional independence. Show more

Keywords: manganese-oxidizing strain, Citrobacter freundii, multicopper oxidase, evolutional independence

DOI: 10.3233/BME-130858

Citation: Bio-Medical Materials and Engineering, vol. 24, no. 1, pp. 703-709, 2014

Authors: Tang, Qiusha | Chen, Daozhen

Article Type: Research Article

Abstract: This paper aimed to investigate the treatment efficiency of 188 Re labeled folate targeting albumin nanoparticles with cis-Diamminedichloroplatinum Cisplatin (188 Re-folate-CDDP/HAS MNP) on human ovarian cancer. SKOV3 cells or tumor-bearing mice were divided into different groups and treated as follow: (A) negative control; (B) chemotherapy; (C) radiotherapy; (D) hyperthermia; (E) chemotherapy and radiotherapy; (F) chemotherapy and hyperthermia; (G) radiotherapy and hyperthermia; (H) chemotherapy, radiotherapy and hyperthermia. Treatment of B to H inhibited proliferation of SKOV3 cells, with the greatest inhibition being observed in group H (P<0.05). Obvious apoptotic hypodiploid peak appeared beside G1 phase in groups of B …to H. The apoptotic rates of SKOV3 cells in groups of A to H were 0.08%, 7.56%, 8.64%, 17.14%, 21.64%, 23.77%, 33.94% and 57.16%, respectively. Our findings in vivo study showed that the mass of tumor in each group of B to H was significantly lower than that in the negative control (p <0.05). In addition, compared with each group of B to G, group H showed highest inhibition of tumor growth (p<0.05). In conclusion, the combination of magnetic induced hyperthermia, chemotherapy and targeted radionuclide of radiation exposure can effectively inhibit the growth of ovarian cancer, which indicates a potential applications in ovarian cancer treatment. Show more

Keywords: Cisplatin, folic acid, albumin magnetic nanoparticles, 188Reovarian cancer, combinated therapy

DOI: 10.3233/BME-130859

Citation: Bio-Medical Materials and Engineering, vol. 24, no. 1, pp. 711-722, 2014

Authors: Kang, Can | Huang, Qifeng | Li, Yunxiao

Article Type: Research Article

Abstract: Rotary blood pump (RBP) is a kind of crucial ventricular assist device (VAD) and its advantages have been evidenced and acknowledged in recent years. Among the factors that influence the operation performance and the durability of various rotary blood pumps, medium property and the flow features in pump's flow passages are conceivably significant. The major concern in this paper is the fluid dynamics aspects of such a kind of miniaturized pump. More specifically, the structural features of axial-flow blood pump and corresponding flow features are analyzed in detail. The narrow flow passage between blade tips and pump casing and the …rotor-stator interaction (RSI) zone may exert a negative effect on the shear stress distribution in the blood flow. Numerical techniques are briefly introduced in view of their contribution to facilitating the optimal design of blood pump and the visualization of shear stress distribution and multiphase flow analysis. Additionally, with the development of flow measurement techniques, the high-resolution, effective and non-intrusive flow measurement techniques catering to the measurement of the flows inside rotary blood pumps are highly anticipated. Show more

Keywords: Axial-flow pump, ventricular assist device, flow, rotor-stator interaction, numerical simulation

DOI: 10.3233/BME-130860

Citation: Bio-Medical Materials and Engineering, vol. 24, no. 1, pp. 723-729, 2014

Authors: Yang, Mingying | Shuai, Yajun | Zhou, Guanshan | Mandal, Namita | Zhu, Liangjun

Article Type: Research Article

Abstract: Antheraea pernyi (A. pernyi) silk fibroin, which is spun from a wild silkworm, has increasingly attracted interest in the field of tissue engineering. The aim of this study was to investigate the nucleation of hydroxyapatite (HAp) on A. pernyi fibroin film. Von Kossa staining proved that A. pernyi fibroin had Ca binding activity. The A. pernyi fibroin film was mineralized with HAp crystals by alternative soaking in calcium and phosphate solutions. Spherical crystals were nucleated on the A. pernyi fibroin film according to scanning electron microscopeimaging results. The FT-IR and X-ray diffraction spectra confirmed that these spherical crystals were HAp. …The results of in vitro cell culture using MG-63 cells demonstrated that the mineralized A. pernyi fibroin film showed excellent cytocompatibility and sound improvement of the MG-63 cellviability. Show more

Keywords: Antheraea pernyi fibroin, hydroxyapatite, mineralization, cell culture

DOI: 10.3233/BME-130861

Citation: Bio-Medical Materials and Engineering, vol. 24, no. 1, pp. 731-740, 2014

Authors: Zhu, Deyi | Jin, Liqiang | Wang, Xuemei | Xu, Li | Liu, Tianqi

Article Type: Research Article

Abstract: The objective of this work was to evaluate the effect of decellularization and hyaluronic acid derivative on the improvement of anticalcification of glutaraldehyde fixed bovine pericardium (GFBP) using a rat subcutaneous implantation model A cell extraction process was employed to remove the cells and cellular components from bovine pericardium (BP), leaving a framework of largely insoluble collagen. Then acellular BP was cross-linked by glutaraldehyde solution and treated with hyaluronic acid derivative (HA-ADH) which was obtained by coupling adipic dihydrazide (ADH) on–COOH of hyaluronic acid (HA). The results of in vivo calcification tests showed that the calcium content was decreased dramatically …by decellularization alone (from 28.07±18.87 to 2.44±0.55μg Ca/mg dry tissue after 8 weeks' implantation), and even less concentration was shown by the combination of HA derivative treatment and decellularization (GFaBP-HA group) (0.25±0.08μg Ca/mg dry tissue after 8 weeks' implantation). In addition, GFaBP-HA group not only presented a lower degree of calcification, but also showed lower ratios of Ca/P molar, which corresponded to amorphous calcium phosphates. The obtained results indicated that GFaBP-HA was a potential candidate for the manufacture of anticalcification bioprostheses. Show more

Keywords: Glutaraldehyde fixed bovine pericardium, anticalcification, decellularization, hyaluronic acid derivative

DOI: 10.3233/BME-130862

Citation: Bio-Medical Materials and Engineering, vol. 24, no. 1, pp. 741-749, 2014

Authors: Wu, Gang | Chen, Long | Li, Hong | Wang, Ying-jun

Article Type: Research Article

Abstract: Hyaluronic acid (HA) was used as an internal phase additive to improve the loading efficiency of ofloxacin, a hydrophilic drug encapsulated by hydrophobic polylactic-co-glycolic acid (PLGA) materials, through a double emulsion (water-in-oil-in-water) solvent extraction/evaporation method. Results from laser distribution analysis show that polyelectrolyte additives have low impact on the average particle size and distribution of the microspheres. The negatively charged HA increases the drug loading efficiency as well as the amount of HA in microspheres. Burst release can be observed in the groups with the polyelectrolyte additives. The release rate decreases with the amount of HA inside the microspheres in …all negatively charged polyelectrolyte-added microsphere groups. Show more

Keywords: Drug release, microsphere, ofloxacin, hyaluronic acid

DOI: 10.3233/BME-130863

Citation: Bio-Medical Materials and Engineering, vol. 24, no. 1, pp. 751-756, 2014

Authors: Liu, Hsia-Wei | Huang, Ching-Cheng

Article Type: Research Article

Abstract: New designed combined products and its determination for pain management and cancer treatment were studied. A rapid and sensitive stability indicating HPLC method had been developed and validated for the determination of Indomethacin(IDM) in a transdermal patch. This analytical method was successfully applied to the determination of Indomethacin in a transdermal patch and can be used for routine quality control analysis. Chromatographic separation was achieved isocratically on an Inertsil® C8-3 column utilizing a mobile phase of acetonitrile / 0.01 M monobasic sodium phosphate and 0.01M dibasic sodium phosphate buffer (pH 3) (65:35, v/v) at the flow rate of 1 mL/min …with UV detection at the wavelength of 210 nm. The system suitability was performed, and the result showed that Indomethacin(IDM) and its impurity were separated. The calibration curve of Indomethacin(IDM) was linear in the range of 0.1~15 ppm (r = 0.9989, n = 3). Show more

Keywords: Controlled release, HPLC, Indomethacin, transdermal route, drug delivery

DOI: 10.3233/BME-130864

Citation: Bio-Medical Materials and Engineering, vol. 24, no. 1, pp. 757-762, 2014

Authors: Sun, Lin | Xu, Jiucheng

Article Type: Research Article

Abstract: Feature selection is a key problem in tumor classification and related tasks. This paper presents a tumor classification approach with neighborhood rough set-based feature selection. First, some uncertainty measures such as neighborhood entropy, conditional neighborhood entropy, neighborhood mutual information and neighborhood conditional mutual information, are introduced to evaluate the relevance between genes and related decision in neighborhood rough set. Then some important properties and propositions of these measures are investigated, and the relationships among these measures are established as well. By using improved minimal-Redundancy-Maximal-Relevancy, combined with sequential forward greedy search strategy, a novel feature selection algorithm with low time complexity …is proposed. Finally, several cancer classification tasks are demonstrated using the proposed approach. Experimental results show that the proposed algorithm is efficient and effective. Show more

Keywords: Feature selection, neighborhood rough set, mutual information, tumor classification

DOI: 10.3233/BME-130865

Citation: Bio-Medical Materials and Engineering, vol. 24, no. 1, pp. 763-770, 2014

Authors: Jung, Gu-In | Kim, Ji-Sun | Lee, Tae-Hee | Choi, Ju-Hyeon | Oh, Han-Byeol | Kim, A-Hee | Eom, Gwang-Moon | Lee, Jeong-Hwan | Chung, Soon-Cheol | Park, Jong-Rak | Lee, Young-Jae | Park, Hee-Jung | Jun, Jae-Hoon

Article Type: Research Article

Abstract: For diagnostic and therapeutic purposes, the joint angle measurement of a patient after an accident or a surgical operation is significant for monitoring and evaluating the recovering process. This paper proposed an optical fiber sensor for the measurement of angular displacement. The effect of beveled fiber angle on the detected light signal was investigated to find an appropriate mathematical model. Beveled fiber tips redirected the light over a range of angles away from the fiber axis. Inverse polynomial models were applied to directly obtain and display the joint angle change in real time with the Lab-VIEW program. The actual joint …angle correlated well with the calculated LabVIEW output angle over the test range. The proposed optical sensor is simple, cost effective, small in size, and can evaluate the joint angle in real time. This method is expected to be useful in the field of rehabilitation and sport science. Show more

Keywords: Optical fiber, Angular sensor, Beveled tip, Asymmetric beam profile, Goniometer

DOI: 10.3233/BME-130867

Citation: Bio-Medical Materials and Engineering, vol. 24, no. 1, pp. 771-780, 2014