Issue title: Papers from the 8th Scientific Meeting on Cartilage Engineering, September 2013, Nancy, France
Article type: Research Article
Authors: Brun, V.; | Guillaume, C.; | Mechiche Alami, S.; | Josse, J.; | Jing, J.; | Draux, F.; | Bouthors, S.; | Laurent-Maquin, D.; ; | Gangloff, S.C.; | Kerdjoudj, H.; | Velard, F.; ; ; ;
Affiliations: EA 4691 «Biomatériaux et Inflammation en Site Osseux», Pôle Santé, SFR CAP-Santé (FED 4231), Université de Reims Champagne-Ardenne, Reims, France | Département de Chirurgie Orthopédique et Traumatologie, CHU de Reims, Reims, France | UFR d'Odontologie, URCA, Reims, France | UFR de Pharmacie, URCA, Reims, France | Département de Chirurgie Dentaire, CHU de Reims, Reims, France | Plateforme d'Imagerie Cellulaire et Tissulaire, URCA, Reims, France
Note: [] Authors have contributed equally to the work.
Note: [] Address for correspondence: Dr. Frédéric Velard, EA 4691 «Biomatériaux et Inflammation en Site Osseux», Pôle Santé, SFR Cap-Santé (FED 4231), Université de Reims Champagne-Ardenne, 51 Rue Cognacq Jay, 51095 Reims Cedex, France. Tel.: +33 3 26 91 80 10; Fax: +33 3 26 91 86 43; E-mail: [email protected]
Abstract: BACKGROUND: To favor regeneration following critical bone defect, a combination of autologous bone graft and biomaterials is currently used. Major drawbacks of such techniques remain the availability of the autologous material and the second surgical site, inducing pain and morbidity. OBJECTIVE: Our aim was to investigate the biocompatibility in vitro of three dimensions hybrid biodegradable scaffolds combining osteoconductive properties of hydroxyapatite and anti-inflammatory properties of chitosan. METHODS: Hybrid scaffolds were characterized by microscopic observations, equilibrium swelling ratio and overtime weight loss measurements. In vitro studies were performed using primary human bone cells cultured for 7, 14 and 21 days. Cell viability, proliferation, morphology and differentiation through alkaline phosphatase (ALP) activity measurement were assessed. RESULTS: Characterization of our scaffolds demonstrated porous, hydrophilic and biodegradable characteristics. In vitro studies showed that these scaffolds have induced slight decrease in cell death and proliferation comparing to the culture plastic substrate control condition, as well as increased short term osteoinductive properties. CONCLUSIONS: In this study, we have provided evidence that our hybrid hydroxyapatite/chitosan scaffolds could be suitable for bone filling.
Keywords: Hydroxyapatite, three dimensions hybrid scaffold, human primary bone cells
DOI: 10.3233/BME-140975
Journal: Bio-Medical Materials and Engineering, vol. 24, no. s1, pp. 63-73, 2014
