Donor's age dependent proliferation decrease of human bone marrow mesenchymal stem cells is linked to diminished clonogenicity

Issue title: Papers from the 8th Scientific Meeting on Cartilage Engineering, September 2013, Nancy, France

Article type: Research Article

Authors: Li, Yueying^; | Charif, Naceur^; | Mainard, Didier^{; ;} | Bensoussan, Danièle^{; ;} | Stoltz, Jean-François^{; ;} | de Isla, Natalia^{; ;}

Affiliations: CNRS, UMR 7365 IMoPA, Vandœuvre-lès-Nancy, France | School of Medicine, Wuhan University, China | Faculty of Medicine, UL, Vandœuvre-lès-Nancy, France | Nancy Hospital, Vandœuvre-lès-Nancy, France

Note: [] Address for correspondence: Natalia de Isla, CNRS, UMR 7365 IMoPA, Vandœuvre-lès-Nancy, France. E-mails: [email protected], [email protected]

Abstract: While mesenchymal stem cells represent an interesting cell source for regenerative medicine, several points have to be investigated to improve their use in clinical, and in particular in the elderly population. This work studied the proliferation capacity of mesenchymal stem cells isolated from human bone marrow in function of donor's age. Doubling time after in vitro culture, clonogenicity and phenotype were analyzed in 17 samples ranging from 3 to 85 years old (mean 47±27). Results showed an increase in the doubling time for cell coming from old donor compared to cells coming from young ones. This was accompanied by a decrease in clonogenicity while no changes were observe in cell phenotype. In conclusion, this study showed an effect of donor's age on the proliferation capacity of mesenchymal stem cells isolated from bone marrow that was correlated to a decrease in clonogenicity. The comprehension of molecular mechanism involved in this process could help to improve the clinical application of mesenchymal stem cells.

Keywords: Mesenchymal stem cells, bone marrow, aging, proliferation, clonogenicity

DOI: 10.3233/BME-140973

Journal: Bio-Medical Materials and Engineering, vol. 24, no. s1, pp. 47-52, 2014