Increased internalization of Staphylococcus aureus and cytokine expression in human Wharton's jelly mesenchymal stem cells

Issue title: Papers from the 8th Scientific Meeting on Cartilage Engineering, September 2013, Nancy, France

Article type: Research Article

Authors: Josse, Jérôme^; | Velard, Frédéric^{; ;} | Mechiche Alami, Saad^; | Brun, Valérie^; | Guillaume, Christine^; | Kerdjoudj, Halima^; | Lamkhioued, Bouchaib^; | Gangloff, Sophie C.^{; ;}

Affiliations: EA 4691 «Biomatériaux et Inflammation en Site Osseux», Pôle Santé, SFR CAP-Santé (FED 4231), Université de Reims Champagne-Ardenne, Reims, France | UFR de Pharmacie, URCA, Reims, France | UFR d'Odontologie, URCA, Reims, France | Plateforme en Imagerie Cellulaire et Tissulaire (PICT), URCA, Reims, France | Département de Chirurgie Orthopédique et Traumatologie, CHU de Reims, Reims, France

Note: [] Address for correspondence: Sophie C. Gangloff, EA 4691 “Bios”, UFR de Pharmacie de Reims, 1 Avenue du Maréchal Juin, 51095 Reims Cedex, France. Tel.: +33 3 26 91 35 95; Fax: +33 3 26 91 86 43; E-mail: [email protected]

Abstract: BACKGROUND: Although a large number of studies have documented the interaction of mesenchymal stem cells (MSCs) with cells of both the innate and adaptive immune systems, not much is known about how bacteria interact with MSCs and how this might influence MSCs behavior. In this study, we investigated the impact of Staphylococcus aureus (S. aureus), on viability and cytokines' production of human Wharton's jelly-MSCs (WJ-MSCs). OBJECTIVE: To investigate if WJ-MSCs: (1) internalize S. aureus; (2) are able to survive and (3) release immunomodulatory mediators after interaction with S. aureus. METHODS: WJ-MSCs were exposed to S. aureus at a multiplicity of infection (MOI) of 10:1 or 30:1 for different designed times. After interaction, intracellular bacteria were quantified as well as MSCs viability. Expression and cytokine-secretion were assessed using quantitative real-time PCR and ELISA. RESULTS: We found that the challenge of WJ-MSCs with S. aureus resulted in increased internalization of S. aureus in a time-dependent manner until six hours post-infection at either MOI of 10:1 and 30:1 and in increased expression of IL-6 mRNA and secretion of TNF-α at six hours and nine hours post-infection (p<0.05). CONCLUSIONS: These results indicate that WJ-MSCs are able to internalize S. aureus and reveal a potential important function of these cells in the immune response.

Keywords: Human Wharton's jelly stem cells (WJ-MSCs), Staphylococcus aureus (S. aureus), inflammation, internalization

DOI: 10.3233/BME-140971

Journal: Bio-Medical Materials and Engineering, vol. 24, no. s1, pp. 27-35, 2014