Licochalcone A inhibiting proliferation of bladder cancer T24 cells by inducing reactive oxygen species production

Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 2nd International Conference on Biomedical Engineering and Biotechnology, 11–13 October 2013, Wuhan, China

Article type: Research Article

Authors: Jiang, Jiangtao | Yuan, Xuan | Zhao, Hong | Yan, Xinyan | Sun, Xiling^; | Zheng, Qiusheng^;

Affiliations: School of Pharmacy, Shihezi University, Shihezi, 832002, Xinjiang, China | School of integrated traditional Chinese and Western Medicine, Binzhou Medical College, Yantai 264000, Shandong, China

Note: [] Address for Correspondence: Binzhou Medical College, Yantai 264000, Shandong, China. Phone: +86 05356886837; fax: +86 005356886837; E-mail addresses: [email protected]; Shihezi University, beierlu, shihezi, China. Phone: +86 09932057003; fax: +86 099320570; E-mail addresses: [email protected].

Note: [] Address for Correspondence: Binzhou Medical College, Yantai 264000, Shandong, China. Phone: +86 05356886837; fax: +86 005356886837; E-mail addresses: [email protected]; Shihezi University, beierlu, shihezi, China. Phone: +86 09932057003; fax: +86 099320570; E-mail addresses: [email protected].

Abstract: The aim of this study was to determine the relationship between proliferation inhibition and the production of reactive oxygen species (ROS) induced by Licochalcone A (LCA). Cell viability was evaluated using sulforhodamine B (SRB) assay. Intracellular ROS level was assessed using the 2, 7-dichlorofluorescein diacetate (H2DCFDA) probe and dihydroethidium (DHE) probe assay. The results indicate that LCA inhibits human bladder cancer T24 proliferation in a concentration-dependent manner, with an IC50 value of approximately 55 μM. The LCA-induced ROS production is inhibited by the co-treatment of LCA and free radical scavenger N-acetyl-cysteine (NAC), on the contrary, the proliferation rate and ROS production increase when treated by the combination of LCA and L-buthionine-(S,R)-sulfoximine (BSO). The ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) decreases in a concentration-dependent manner. The results suggest that LCA inhibits proliferation by increasing intracellular ROS levels resulted in an oxidative stress status in T24 cells.

Keywords: Licochalcone A, T24 cells, ROS, Cell proliferation, GSH, GSSG

DOI: 10.3233/BME-130899

Journal: Bio-Medical Materials and Engineering, vol. 24, no. 1, pp. 1019-1025, 2014