Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 2nd International Conference on Biomedical Engineering and Biotechnology, 11–13 October 2013, Wuhan, China
Article type: Research Article
Authors: Yang, Yueh-Lung | Chang, Ching-Hsien | Huang, Ching-Cheng; | Kao, Wenny Mei-Wen; | Liu, Wei-Chung | Liu, Hsia-Wei;
Affiliations: Department of Traditional Chinese Medicine, Chang Gung Memorial Hospital, Keelung, Taiwan | Department of Chinese Medicine, En Chu Kong Hospital, New Taipei City, Taiwan | Department of Biomedical Engineering, Ming-Chuan University, Taoyuan, Taiwan | Regional R&D Service Dep., Metal Industries Research & Development Centre, Taipei, Taiwan | Institute of Plant Biology, National Taiwan University, Taipei, Taiwan | MesoPhase Technologies Inc., Taiwan | Department of Life Science, Fu Jen Catholic University, New Taipei City, Taiwan
Note: [] Address for correspondence: Hsia-Wei Liu, Department of Life Science, Fu Jen Catholic University, No.510, Jhongiheng Rd., Sinjhuang Dist., New Taipei City 24205, Taiwan. Tel.: +886-2-29053740; Fax: +886-2-29052193; E-mail: [email protected]
Abstract: Numerous materials have been proposed for bone tissue engineering. In this study, a newly designed hybrid composite scaffold composed of poly (D,L-lactide-co-glycolide) and a naturally bioceramic hybrid material, nanonized pearl powder, were prepared and the biological activities and physical properties of the scaffold for bone tissue engineering were evaluated. It is a composite consisting calcium carbonate crystal in an aragonite structure, embedded in an organic matrix. Peral contains one or more signal molecules capable of stimulating bone formation. The nanonized pearl powder is considered as a promising osteoinductive biomaterial. This biomaterial is biocompatible and shows osteogenic activity. In this study, the designed biohybrid of nanonized pearl powder/poly (lactide-co-glycolide) (NPP/PLGA) biocomposite scaffolds would employ biodegradable material as MC3T3-E1 cells seeded scaffolds. Therefore, the biocomposite scaffolds would be used to culture with MC3T3-E1 cells under spinner bioreactor in vitro. Furthermore, it also detailed how these tissues were characterized, qualitatively and quantitatively, with scanning electron microscopy and biochemical testing. The identity and the mode of action of these molecules on the osteoblast differentiation were analyzed. This study indicates that the efficiency of nanonized pearl powders in bone cell differentiation are certainly different from that of proteins. Further sudy will look forward to manufacturing the promising new generation bone substitute, three dimensional biocomposite scaffolds to replace the implant and autogeneous bone graft, which combines basic research and clinical application.
Keywords: nanonized pearl powder, poly (D, L-lactide-co-glycolide), osteoblast, bio-mineralization, bone tissue engineering
DOI: 10.3233/BME-130893
Journal: Bio-Medical Materials and Engineering, vol. 24, no. 1, pp. 979-985, 2014
