Affiliations: Department of Orthopedic Surgery, Martin Luther University Halle–Wittenberg, Halle (Saale), Germany | Department of Trauma Surgery, Employers' Liability Insurance Association Hospital Bergmannstrost, Halle (Saale), Germany | Department of Trauma Surgery, Friedrich Schiller University Jena, Jena, Germany | Federal Institute for Materials Research and Testing, Berlin, Germany | IZM, Nanotechnikum Weinberg, Martin Luther University Halle–Wittenberg, Halle (Saale), Germany | Department of Orthopedic and Trauma Surgery, Albert Ludwig University Freiburg, Freiburg, Germany
Note: [] Address for correspondence: Dr. Florian Radetzki, MD, Department of Orthopedic Surgery, Martin Luther University Halle–Wittenberg, Magdeburger Strasse 22, 06112 Halle, Germany. Tel.: +49 345 557 4805/4804; E-mail: [email protected].
Abstract: Resorbable ceramics can promote the bony integration of implants. Their rate of degradation should ideally be synchronized with bone regeneration. This study examined the effect of rapidly resorbable calcium phosphate ceramics 602020, GB14, 305020 on adherence, proliferation and morphology of human bone-derived cells (HBDC) in comparison to β-TCP. The in vitro cytotoxicity was determined by the microculture tetrazolium (MTT) assay. HBDC were grown on the materials for 3, 7, 11, 15 and 19 days and counted. Cell morphology, cell attachment, cell spreading and the cytoskeletal organization of HBDC cultivated on the substrates were investigated using laser scanning microscopy and environmental scanning electron microscopy. All substrates supported sufficient cellular growth for 19 days and showed no cytotoxicity. On each material an identical cell colonisation of well communicating, polygonal, vital cells with strong focal contacts was verified. HBDC showed numerous well defined stress fibres which give proof of well spread and strongly anchored cells. Porous surfaces encouraged the attachment and spreading of HBDC. Further investigations regarding long term biomaterial/cell interactions in vitro and in vivo are required to confirm the utility of the new biomaterials.
