Different effects of nanophase and conventional hydroxyapatite thin films on attachment, proliferation and osteogenic differentiation of bone marrow derived mesenchymal stem cells
Affiliations: Huzhou Key Laboratory of Molecular Medicine, Huzhou Central Hospital, Huzhou, Zhejiang Province, China | Department of Biotechnology, College of Life Science, Zhe Jiang University, China | Department of Chemistry, College of Science, Zhe Jiang University, China
Note: [] Addresses for correspondence: Ming Zhang, 325 Room, College of Life Science, Zi Jin Gang Campus, Zhe Jiang University, Hangzhou, 310058, China. Tel.: +86 0571 88206659; E-mail: [email protected]
Abstract: It is suggested that nanophase hydroxyapatite (nHAP) might have advantages over conventional hydroxyapatite (cHAP) as a biomaterial for bone regeneration. To be a satisfactory candidate for bone tissue engineering, it is important to support the growth and differentiation of bone marrow-derived mesenchymal stem cells (BMSCs). The purpose of this study is to determine whether nHAP as cell growth substrata could give better support for attachment, proliferation and osteogenic differentiation of BMSCs than cHAP. Materials and Methods: nHAP and cHAP films were prepared as the substrata for the cell growth. BMSCs obtained from rabbit were seeded on the films. Attachment, proliferation and osteogenic differentiation of BMSCs on the two kinds of films were evaluated. Results: Cell attachment ratio on nHAP films was significantly higher than that on cHAP films. Doubling time on nHAP films was significantly shorter than that on cHAP films. Amount of total proteins detected from cells cultured on nHAP films was significantly higher than that on cHAP films. However, alkaline phosphatase activity and osteocalcin content of the two groups showed no significant difference. Conclusions: nHAP films favored cell attachment and proliferation but not osteogenic differentiation of BMSCs compared with cHAP films.
