Affiliations: Laboratoire de Mécanique et Ingénierie Cellulaire et Tissulaire, LEMTA-UMR 7563 (CNRS-UHP-INPL) et IFR 111, Faculté de Médecine, 54505 Vandoeuvre-lès-Nancy, France | INSERM U 595, Faculté de Chirurgie Dentaire, 67085 Strasbourg, France | Institut Charles Sadron (CNRS-ULP), UPR 22 CNRS, 67083 Strasbourg, France
Note: [] Corresponding author: C. Boura, Laboratoire de Mécanique et Ingénierie Cellulaire et Tissulaire, LEMTA-UMR 7563 (CNRS-UHP-INPL) et IFR 111, Faculté de Médecine, 54505 Vandoeuvre-lès-Nancy, France. Tel.: +33 3 83 68 34 74; Fax: +33 3 83 68 34 59; E-mail: [email protected].
Abstract: Polyelectrolyte multilayer films were recently investigated to favour attachment of Human Vein Umbilical Endothelial Cells (HUVECs) on non-adhesive surfaces. In this study, we evaluated the initial adhesion of HUVECs after 3 h of seeding on two polyelectrolyte multilayer films ending by poly(D-lysine) (PDL) or poly(allylamine hydrochloride) (PAH). In order to obtain information about initial adhesion of HUVECs, cell morphology as well as the expression of β1 integrins, specific receptors of adhesion, were evaluated after 3 h of seeding on polyelectrolyte multilayer films. The data were also compared to PDL or PAH monolayers (polyelectrolytes terminating the multilayer architecture). The expression of β1 integrins was not different, whatever are the studied surfaces. However, HUVECs spreading on polyelectrolyte multilayer films, in particular on PAH ending film, was more important as compared to polyelectrolyte monolayers or glass. In conclusion, the best initial adhesion conditions of HUVECs on polyelectrolyte films could not be elucidated, moreover the results suggested also that β1 integrins could only play a limited role.
