Affiliations: Banque de Tissus et Cellules des Hospices Civils de Lyon, Hôpital E. Herriot, 69437 Lyon, Rhône, France | Laboratoire d'immunologie, Centre Hospitalier de Lyon sud, Saint Genis Laval, Rhône, France | Centre de traitement des brûlés, Hopsices Civils de Lyon, Hôpital E. Herriot, 69437 Lyon, Rhône, France | Service de dermatologie, Pavillon R, Hôpital E. Herriot, 69437 Lyon, Rhône, France
Note: [] Corresponding author: Odile Damour, Banque de Tissus et Cellules des Hospices Civils de Lyon, Hôpital E. Herriot, 69437 Lyon, Rhône, France. Tel.: +33 4 72 11 75 81; Fax: +33 4 72 11 62 14.
Abstract: The clinical interest of using allogenic epidermal sheets (AES) has largely been shown [1,2,3]. As well as covering, they also stimulate healing, by simultaneously secreting numerous growth factors (GFs), although little is known on their mechanism of action. Our objectives were to: (a) devise a test for the efficacy of AES release, (b) select keratinocyte-secreting strains and optimal culture conditions. Three GFs were selected: IL-1α, IL-8 and VEGF. Three different keratinocyte strains were cultured for 3 and 6 days after confluence for 3 passages. Assays were performed after 3 h and 24 h+3 h after dispase treatment (AES conservation for 24 h then change of medium and sampling after 3 h). AES were found to secrete GFs in DMEM and the amounts were greater when cultured for 6 rather than 3 days after confluence. Each strain had different secretory patterns depending on passage and time in culture, this variability being explained by inter-individual heterogeneity.
