Affiliations: [a] Basic Science Laboratory, Faculty of Stomatology, San Luis Potosí University, San Luis Potosi, Mexico | [b] Biochemical and Microbiology Laboratory, Faculty of Stomatology, San Luis Potosí University, San Luis Potosi, Mexico | [c] Paediatric Dentistry Postgraduate Program, Faculty of Stomatology, San Luis Potosí University, San Luis Potosi, Mexico | [d] Faculty of Stomatology, San Luis Potosí University, San Luis Potosi, Mexico
Correspondence:
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Corresponding author: Marine Ortiz-Magdaleno, Basic Science Laboratory, Faculty of Stomatology, San Luis Potosí University, Av. Dr. Manuel Nava #2, Zona Universitaria, 78290, San Luis Potosí, SLP, Mexico. E-mail: [email protected]
Abstract: BACKGROUND:Acrylic resins used in dental and biomedical applications do not have antimicrobial properties, their surface is susceptible to colonization of microorganisms. OBJECTIVE:The aim of this study was to evaluate the antibiofilm properties of silver nanoparticles (AgNPs) deposited in a polymethyl methacrylate (PMMA) surface against a Staphylococcus aureus biofilm. METHODS:The PMMA was impregnated with AgNPs by using the in-situ polymerization method. To determine the solubility of the incorporated silver (Ag+) atomic absorption spectrophotometry was used (AAS) at 24 h, 48 h, 7 days, and 30 days. Thirty specimens of PMMA with AgNPs and without NP (control group) were assembled in the CDC Biofilm Bioreactor system with a cell suspension of S. aureus. The specimens were removed at 6, 12, 24, 48, and 72 h to determine the viability profile and quantify the Arbitrary Fluorescence Units (AFU). RESULTS:The AgNPs showed an irregular and quasispherical shape with an average size of 25 nm. AAS analysis demonstrated a low solubility of Ag+. The formation of the S. aureus biofilm increased as the evaluation periods continued up to 72 h. The experimental group showed poor growth, and a decrease in the intensity of the fluorescence demonstrated a statistically significant inhibition of the formation of the biofilm (P < 0.05) in relation to the control group at 6, 12, 24, 48, and 72 h. CONCLUSION:AgNPs incorporated into PMMA decreased the growth and maturation of S. aureus biofilm.
