Decellularized liver-regenerative 3D printing biomaterials for cell-based liver therapies via a designed procedure combining supercritical fluids with papain-containing reagents

Article type: Research Article

Authors: Huang, Ching-Cheng^{a; b;}

Affiliations: [a] Department of Biomedical Engineering, Ming-Chuan University, Taoyuan, Taiwan | [b] PARSD Biomedical Material Research Center, Taichung, Taiwan

Correspondence: [*] E-mail: [email protected]

Abstract: BACKGROUND:The biologic scaffolds derived from decellularized tissues and organs have been successfully developed in a variety of preclinical and/or clinical studies. OBJECTIVE:The new decellularized liver-regenerative 3D printing biomaterials were designed and prepared for cell-based liver therapies. METHODS:An extraction process was employed to remove the tissue and cellular molecules from porcine liver via pretreatment of supercritical fluid of carbon dioxide (ScCO2). Varying porosities of the decellularized liver tissues were created using papain-containing reagent treatments after ScCO2. RESULTS:The resulting liver-regenerative 3D printing biomaterials of decellularized liver collagen scaffolds were characterized by Fourier transform infrared spectroscopy, thermo-gravimetric analysis, differential scanning calorimetry and scanning electron microscopy. CONCLUSIONS:The decellularized liver collagen scaffolds with good thermal stability (>150 °C) were obtained and employed as liver-regenerative 3D printing biomaterials for cell-based liver therapies.

Keywords: Supercritical fluid, decellularization, scaffold, morphology

DOI: 10.3233/BME-211303

Journal: Bio-Medical Materials and Engineering, vol. 33, no. 2, pp. 139-146, 2022