Affiliations: [a] Department of Ophthalmology, Affiliated Eye Hospital of Nanchang University, China | [b] The Graduate School of Nanchang University, China | [c] Department of Stomatology, The Third Affiliated Hospital of Nanchang University, China | [d] College of Chemistry, Nanchang University, China | [e] Department of Prosthodontics, Affiliated Stomatological Hospital of Nanchang University, China
Correspondence:
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Corresponding author: Fubao Zhang, Department of Stomatology, The Third Affiliated Hospital of Nanchang University, 128 Xiangshan North Road, Nanchang, Jiangxi Province, China. Tel.: +86 15679107727; Fax: +86 079188862303; E-mail: [email protected]
Abstract: BACKGROUND:Craniofacial soft-tissue defects mostly have an impact on the treatment of various oral diseases. Tissue expander is an important technique for tissue reconstruction, especially for soft tissues in reconstructive surgery. OBJECTIVE:This research aimed to develop a new self-swelling tissue expander, namely hydrogel, for soft tissue reconstruction in craniofacial region. METHODS:In vitro, the chemical and physical characteristics of hydrogel were evaluated by SEM, swelling rate, mechanical testing, EDS, and FT-IR. In vivo, the craniofacial implant model of SD rats were divided into group A as control, group B with hydrogels for 1 week expansion, group C for 2 weeks and group D for 4 weeks (n = 5), and the effects were analyzed by HE staining, histological and radiographic evaluation. RESULTS:The in vitro results suggested that dry hydrogel possessed a uniform surface with micropores, the surface of post-swelling hydrogel formed three-dimensional meshwork. Within 24 hours, hydrogels expanded markedly, then slowed down. The mechanical property of hydrogels with longer expansion was better, whose main elements were carbon and oxygen. FT-IR also verified its molecular structure. In vivo, the wounds of rats recovered well, hydrogels could be removed as one whole piece with original shape and examined by radiographic evaluation, besides, the expanded skin and developed fibrous capsule formed surrounding hydrogels. CONCLUSION:The new expander was designed successfully with good chemical and physical characteristics, and could be applied in an animal model to help tissue reconstruction.
Keywords: Tissue expander, biomaterials, rat, tissue reconstruction
