Searching for just a few words should be enough to get started. If you need to make more complex queries, use the tips below to guide you.
Article type: Research Article
Authors: Ghazi Zadeh, Leilia | Chevrier, Anikb | Lamontagne, Martinc | Buschmann, Michael D.d | Hoemann, Caroline D.d | Lavertu, Marcb;
Affiliations: [a] Biomedical Engineering Institute, Polytechnique Montreal, Montreal, QC, Canada | [b] Department of Chemical Engineering, Polytechnique Montreal, Montreal, QC, Canada | [c] Faculty of Medicine, University of Montreal, Montreal, QC, Canada | [d] Department of Bioengineering, Institute for Advanced Biomedical Research, George Mason University, Manassas, VA, USA
Correspondence: [*] Corresponding author: Marc Lavertu, Chemical Engineering Department, Polytechnique Montreal, PO Box 6079, succ. Centre-Ville, Montreal, Quebec, H3C 3A7, Canada. Tel.: 514 340 4711 ext. 3609; Fax: 514 340 2980; E-mail: [email protected]
Abstract: BACKGROUND:Platelet-rich plasma (PRP) has been used to solubilize freeze-dried chitosan (CS) formulations to form injectable implants for tissue repair. OBJECTIVE:To determine whether the in vitro performance of the formulations depends on the type of PRP preparation used to solubilize CS. METHODS:Formulations containing 1% (w/v) CS with varying degrees of deacetylation (DDA 80.5–84.8%) and number average molar mass (Mn 32–55 kDa), 1% (w/v) trehalose and 42.2 mM calcium chloride were freeze-dried. Seven different PRP preparations were used to solubilize the formulations. Controls were recalcified PRP. RESULTS:CS solubilization was achieved with all PRP preparations. CS-PRP formulations were less runny than their corresponding PRP controls. All CS-PRP formulations had a clotting time below 9 minutes, assessed by thromboelastography, while the leukocyte-rich PRP controls took longer to coagulate (>32 min), and the leukocyte-reduced PRP controls did not coagulate in this dynamic assay. In glass culture tubes, all PRP controls clotted, expressed serum and retracted (43–82% clot mass lost) significantly more than CS-PRP clots (no mass lost). CS dispersion was homogenous within CS-PRP clots. CONCLUSIONS:In vitro performance of the CS-PRP formulations was comparable for all types of PRPs assessed.
Keywords: Chitosan, platelet-rich plasma, injectable implants
DOI: 10.3233/BME-191058
Journal: Bio-Medical Materials and Engineering, vol. 30, no. 4, pp. 349-364, 2019
IOS Press, Inc.
6751 Tepper Drive
Clifton, VA 20124
USA
Tel: +1 703 830 6300
Fax: +1 703 830 2300
[email protected]
For editorial issues, like the status of your submitted paper or proposals, write to [email protected]
IOS Press
Nieuwe Hemweg 6B
1013 BG Amsterdam
The Netherlands
Tel: +31 20 688 3355
Fax: +31 20 687 0091
[email protected]
For editorial issues, permissions, book requests, submissions and proceedings, contact the Amsterdam office [email protected]
Inspirees International (China Office)
Ciyunsi Beili 207(CapitaLand), Bld 1, 7-901
100025, Beijing
China
Free service line: 400 661 8717
Fax: +86 10 8446 7947
[email protected]
For editorial issues, like the status of your submitted paper or proposals, write to [email protected]
如果您在出版方面需要帮助或有任何建, 件至: [email protected]