Minoxidil-encapsulated poly(L-lactide-co-glycolide) nanoparticles with hair follicle delivery properties prepared using W/O/W solvent evaporation and sonication

Article type: Research Article

Authors: Takeuchi, Issei^{a; b; c} | Hida, Yukari^a | Makino, Kimiko^{a; b; c; *}

Affiliations: [a] Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641, Yamazaki, Noda, Chiba 278-8510, Japan | [b] Center for Drug Delivery Research, Tokyo University of Science, 2641, Yamazaki, Noda, Chiba 278-8510, Japan | [c] Center for Physical Pharmaceutics, Tokyo University of Science, 2641, Yamazaki, Noda, Chiba 278-8510, Japan

Correspondence: [*] Corresponding author: Kimiko Makino, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641, Yamazaki, Noda, Chiba 278-8510, Japan. Tel.: +81-4-7121-3662; Fax: +81-4-7121-3662; E-mail: [email protected].

Abstract: BACKGROUND:Nanoparticles with a diameter of 100–150 nm prepared using poly(lactide-co-glycolide) can be delivered to hair follicles. Moreover, it was shown that drug release from nanoparticles was prolonged by using poly(L-lactide-co-glycolide) (PLLGA). OBJECTIVE:The aim of this study was to prepared minoxidil-encapsulated PLLGA nanoparticles and accumulate minoxidil in hair follicles utilizing its drug delivery properties for effective treatment of male and female androgenetic alopecia. METHODS:Minoxidil-encapsulated PLLGA nanoparticles were prepared using W/O/W solvent evaporation and sonication, and the ability of PLLGA nanoparticles to deliver to hair follicles was evaluated by in vivo transdermal delivery study using C3H/He mice and tape stripping and cyanoacrylate skin surface biopsy. RESULTS:Eight hours after administration of samples, minoxidil-encapsulated PLLGA nanoparticles delivered 3.1 times more minoxidil in stratum corneum and 2.5 times more in hair follicles r compared to minoxidil aqueous solution. In addition, we found that 4.8% of the dose of minoxidil-encapsulated PLLGA nanoparticles were delivered to the hair follicles. CONCLUSIONS:In this study, it was shown that nanoparticulation using PLLGA7510 suppressed minoxidil release from nanoparticles and improved drug delivery amount to hair follicles.

Keywords: Poly(lactide-co-glycolide), minoxidil, androgenetic alopecia, hair follicles, tape stripping, cyanoacrylate skin surface biopsy

DOI: 10.3233/BME-171724

Journal: Bio-Medical Materials and Engineering, vol. 29, no. 2, pp. 217-228, 2018