Different methods to determine the encapsulation efficiency of protein in PLGA nanoparticles

Article type: Research Article

Authors: Amini, Yousef^{a; b; c} | Amel Jamehdar, Saeid^b | Sadri, Keyvan^d | Zare, Sirwan^{c; g} | Musavi, Danial^e | Tafaghodi, Mohsen^{f; *}

Affiliations: [a] Department of Microbiology, Faculty of Medicine, Zahedan University of Medical Science, Zahedan, Iran | [b] Microbiology & Virology Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran | [c] Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran | [d] Nuclear Medicine Research Center, Mashhad University of Medical Sciences, Mashhad, Iran | [e] Department of Physical Chemistry, Faculty of Chemistry, University of Kashan, Iran | [f] Nanotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran | [g] Department of Immunology, Mashhad University of Medical Sciences, Mashhad, Iran

Correspondence: [*] Corresponding author. Tel.: +98 513 8823252; Fax: +98 513 8823251; E-mail: [email protected].

Abstract: Background:Effective encapsulation of drugs into the delivery systems could increase the efficiency of nanoparticles in prevention and treatment of diseases. Objective:The purpose of this study was to compare the different methods for determination of encapsulation efficiency of a model protein in the PLGA nanoparticles. Methods:The various direct methods include dichloromethane, acetonitrile, modified acetonitrile and NaOH based extraction and radioactive methods were used to directly calculate the encapsulation efficiency of the loaded protein in the PLGA nanoparticles. Furthermore, indirect methods include BCA, Fluorescent and radioactive methods were compared. Results:The encapsulation efficiencies determined by indirect methods include dichloromethane, acetonitrile, modified acetonitrile, NaOH based extraction and radioactive methods were 12.62% ± 1.97, 17.43% ± 2.51, 64.69% ± 4.31, 86.36% ± 2.25 and 90.15% ± 1.78, respectively. Moreover, the encapsulation efficiencies determined by indirect methods include BCA, fluorescent and radioactive methods were 81.46% ± 1.92, 88.23% ± 1.15 and 89.6% ± 1.9, respectively. Conclusions:Among the results obtained by indirect methods, radioactive and fluorescent methods showed more reliable. Moreover, NaOH and radioactive methods were the most reliable methods among the direct methods.

Keywords: Encapsulation efficiency, direct methods, indirect methods, PLGA, nanoparticles

DOI: 10.3233/BME-171705

Journal: Bio-Medical Materials and Engineering, vol. 28, no. 6, pp. 613-620, 2017