Affiliations: [a] Department of Life Science and Applied Chemistry, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan | [b] Department of Physical Science and Engineering, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan
Abstract: Background:In our earlier work, tobermorite containing calcium phosphate (CP) clusters (CP-Tob) was hydrothermally prepared in the CaO–SiO2–P2O5–H2O system for biomedical applications. Objective:CP-Tob was used to investigate the influence of CP cluster incorporation on its biocompatibility. Methods:Tobermorite samples with and without CP clusters were hydrothermally prepared at 180°C for 40 h. The biocompatibility, structure, and density of states of the tobermorite samples were investigated by experimental and first principles methods. Results:The amounts of lysozyme and bovine serum albumin adsorbed on CP-Tob were higher than those on tobermorite without CP clusters. Cluster incorporation caused a decrease in the solubility, resulting in the enhancement of the cell compatibility. The calculated results indicated that incorporating clusters, which interact with the silicate units of tobermorite, led to a change of the density of states of tobermorite. Conclusions:Incorporation of CP clusters in tobermorite led to improvement of the biocompatibility evaluated by biological and computational analyses.
