Hybrid collagen sponge and stem cells as a new combined scaffold able to induce the re-organization of endothelial cells into clustered networks

Issue title: Selected papers from the 6th China–France International Symposium “Stem Cells and Regenerative Medicine” and the first meeting of the France–China International CNRS Network (GDRI) CeSMeR, Nancy, 10–13 July 2016

Guest editors: J.-F. Stoltz, J. Magdalou and D. Bensoussan

Article type: Research Article

Authors: Offner, Damien^{a; b; c; *} | Wagner, Quentin^{a; b; *} | Idoux-Gillet, Ysia^{a; b} | Gegout, Hervé^b | Ferrandon, Arielle^{a; b} | Schwinté, Pascale^{a; b} | Musset, Anne-Marie^{b; c} | Benkirane-Jessel, Nadia^{a; b; **} | Keller, Laetitia^{a; b}

Affiliations: [a] INSERM (French National Institute of Health and Medical Research), ‘Osteoarticular and Dental Regenerative Nanomedicine’ Laboratory, UMR 1109, Faculté de Médecine, F-67085 Strasbourg cedex, FMTS, France | [b] Université de Strasbourg, Faculté de Chirurgie Dentaire, 8 rue Sainte Elisabeth, F-67000 Strasbourg, France | [c] Hôpitaux Universitaires de Strasbourg (HUS), 1 place de l’Hôpital, F-67000 Strasbourg, France

Correspondence: [**] Corresponding author. Tel.: +33 368853376; E-mail: [email protected].

Note: [*] These authors have equally contributed to this work.

Abstract: The time needed to obtain functional regenerated bone tissue depends on the existence of a reliable vascular support. Current techniques used in clinic, for example after tooth extraction, do not allow regaining or preserving the same bone volume. Our aim is to develop a cellularized active implant of the third generation, equipped with human mesenchymal stem cells to improve the quality of implant vascularization. We seeded a commercialized collagen implant with human mesenchymal stem cells (hMSCs) and then with human umbilical vein endothelial cells (HUVECs). We analyzed the biocompatibility and the behavior of endothelial cells with this implant. We observed a biocompatibility of the active implant, and a re-organization of endothelial cells into clustered networks. This work shows the possibility to develop an implant of the third generation supporting vascularization, improving the medical care of patients.

Keywords: Collagen implant, vascularization, mesenchymal stem cells, endothelial cells

DOI: 10.3233/BME-171640

Journal: Bio-Medical Materials and Engineering, vol. 28, no. s1, pp. S185-S192, 2017