Evaluation of the pro-angiogenic effect of nanoscale extracellular vesicles derived from human umbilical cord mesenchymal stem cells

Issue title: Selected papers from the 6th China–France International Symposium “Stem Cells and Regenerative Medicine” and the first meeting of the France–China International CNRS Network (GDRI) CeSMeR, Nancy, 10–13 July 2016

Guest editors: J.-F. Stoltz, J. Magdalou and D. Bensoussan

Article type: Research Article

Authors: Dostert, G.^{a; *} | Willemin, A.-S.^{a; *} | Jouan-Hureaux, V.^b | Louis, H.^{c; d} | Hupont, S.^c | Gillet, P.^{a; e} | Menu, P.^{a; f} | Decot, V.^{a; e} | Moby, V.^{a; e; §} | Velot, É.^{a; f; §; **}

Affiliations: [a] UMR 7365 CNRS-Université de Lorraine IMoPA, Vandœuvre-lès-Nancy, France | [b] UMR 7039 CNRS-Université de Lorraine CRAN, Vandœuvre-lès-Nancy, France | [c] FR BMCT 3209, CNRS-INSERM-UL-CHRU de Nancy, Vandœuvre-lès-Nancy, France | [d] INSERM U1116, Vandœuvre-lès-Nancy, France | [e] CHRU de Nancy, Vandœuvre-lès-Nancy, France | [f] Faculté de Pharmacie, Nancy, France

Correspondence: [**] Corresponding author: É. Velot, UMR 7365 CNRS-Université de Lorraine, Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), Vandœuvre-lès-Nancy, 54505, France. Tel.: +33 3 72 74 65 81; Fax: +33 3 72 74 65 45; E-mail: [email protected].

Note: [*] These authors contributed equally to this work.

Note: [§] These authors jointly directed this work.

Abstract: Mesenchymal stem cells (MSCs) are a common tool in regenerative medicine. The nanoscale extracellular vesicles (nEVs) secreted by these cells were recently brought up to light thanks to their therapeutic potential. In this study, we assessed the in vitro behaviour of human umbilical vein endothelial cells (HUVECs) exposed to nEVs derived from human umbilical cord mesenchymal stem cells (hUC-MSCs). Nanoscale extracellular vesicles were isolated and characterized by NanoSight® and flow cytometry. HUVECs were stimulated with various concentrations of nEVs. To assess nEV interactions with HUVECs, confocal microscopy and angiogenesis assay were performed. The use of nEVs derived from hUC-MSCs was able to produce positive outcomes on HUVECs by acting on their angiogenic potential.

Keywords: Nanoscale extracellular vesicles, HUVECs, human umbilical cord MSCs, angiogenesis

DOI: 10.3233/BME-171626

Journal: Bio-Medical Materials and Engineering, vol. 28, no. s1, pp. S75-S79, 2017