Cellulose/soy protein isolate composite membranes: Evaluations of in vitro cytocompatibility with Schwann cells and in vivo toxicity to animals
Issue title: Selected Papers from the 5th China–France International Symposium “Stem Cells: From Bench to Bedside”, 12–14 December 2013, Kunming, China
Affiliations: Department of Biomedical Engineering, School of Basic Medical Science, Wuhan University, Wuhan, China | School of Medicine, Hubei University of Arts and Sciences, Xiangyang, China | Department of Neurology, Xiangyang Central Hospital affiliated to Hubei University of Arts and Sciences, Xiangyang, China | UMR 7365 CNRS – Université de Lorraine, Ingénierie Moléculaire et Physiopathologie Articulaire, Biopôle, Vandœuvre-lès-Nancy, France
Note: [] Address for correspondence: Yinping Li, Department of Biomedical Engineering, School of Basic Medical Science, Wuhan University, Wuhan 430071, China. Tel.: +86 27 68759509; E-mail: [email protected]
Abstract: OBJECTIVE: To evaluate the in vitro cytocompatibility of cellulose/soy protein isolate composite membranes (CSM) with Schwann cells and in vivo toxicity to animals. METHODS: A series of cellulose/soy protein isolate composite membranes (CSM) were prepared by blending, solution casting and coagulation process. The cytocompatibility of the CSM to Schwann cells were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and by direct cells culture of Schwann cells on the surfaces of the CSM, respectively. The in vivo toxicity of the CSM to animals were also evaluated by acute toxicity testing, skin sensitization testing, pyrogen testing and intracutaneous stimulation testing, respectively, according to the ISO 10993 standard. RESULTS: The MTT assay showed that the cell viability of Schwann cells cultured in extracts from the CSM was higher than that from the neat cellulose membrane without containing SPI component. The direct cells culture indicated that the Schwann cells could attach and grow well on the surface of the CSM and the incorporation of SPI into cellulose contributed to improvement of cell adhesion and proliferation. The evaluations of in vivo biological safety suggested that the CSM showed no acute toxicity, no skin sensitization and no intracutaneous stimulation to the experimental animals. CONCLUSION: The CSM had in vitro cytocompatibility with Schwann cells and biological safety to animals, suggesting potential for the applications as nerve conduit for the repair of nerve defect.
Keywords: Cellulose, soy protein isolate, nerve conduit, cytocompatibility, acute toxicity
