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Mouse colorectal cancer an early detection approach using nonlinear microscopy

Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 3rd International Conference on Biomedical Engineering and Biotechnology, 25–28 September 2014, Beijing, China

Article type: Research Article

Authors: Bianchi, Mariana; | Adur, Javier; | Ruff, Silvana Y. | Izaguirre, María F. | Carvalho, Hernandes F. | Cesar, Carlos L. | Casco, Víctor H.;

Affiliations: Microscopy Laboratory Applied to Molecular and Cellular Studies, Engineering School, National University of Entre Rios, (3101) Oro Verde, Entre Rios, Argentina | Research and Transfer Center of Entre Ríos (CITER) CONICET-UNER (3101) Oro Verde. Entre Ríos. Argentina | INFABiC – National Institute of Science and Technology on Photonics Applied to Cell Biology, Campinas, São Paulo, Brazil

Note: [] These authors contributed equally to this work.

Note: [] Corresponding author: Víctor H. Casco, Microscopy Laboratory Applied to Molecular and Cellular Studies, Engineering School, National University of Entre Rios, (3101) Oro Verde, Entre Rios, Argentina. E-mail: [email protected].

Keywords: Colon cancer, early detection, second harmonic generation, two-photon excitation fluorescence

DOI: 10.3233/BME-141166

Journal: Bio-Medical Materials and Engineering, vol. 24, no. 6, pp. 3419-3426, 2014

Published: 2014
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Abstract

Murine induced colon cancer has been used to demonstrate that Second Harmonic Generation (SHG) microscopy images, combined with Two-Photon Excitation Fluorescence (TPEF) and specific quantization scoring methods allow distinguishing early alterations in colon mucosa. TPEF was used only to identified crypts and submucosa regions, whereas the image analysis was used to get quantitative data (Integrated Intensity and Aspect Ratio scoring) of different cancer stages. The submucosa amount of collagen fibers was significant and their orientation suffering proportional changes with the development of the pathological processes. Both after the fourth and eighth weeks after colon cancer induction, integrated intensity and aspect ratio values have shown significant statistical differences compared with control samples. Thus, SHG microscopy has proved to be a useful quantitative tool to highlight early changes of submucosa and the progression of these through the cancer development.

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