Affiliations: Thermal Spray Centre, Universitat de Barcelona, Barcelona, Spain | URFOA, Institut Hospital del Mar d'Investigacions Mèdiques, RETICEF, Barcelona, Spain | Servei de Medicina Interna, Hospital del Mar, Barcelona, Spain
Note: [] Address for correspondence: Hortensia Melero, Thermal Spray Centre, Universitat de Barcelona, Martí i Franqués, 1, 08028 Barcelona, Spain. Tel.: +34 603 823 033; Fax: +34 93 402 16 38; E-mail: [email protected]
Abstract: Hydroxyapatite coatings obtained by plasma-spraying have been used for many years to improve biological performance of bone implants, but several studies have drawn attention to the problems arising from high temperatures and the lack of mechanical properties. In this study, plasma-spraying is substituted by high velocity oxy-fuel (HVOF) spray, with lower temperatures reached, and TiO2 is added in low amounts to hydroxyapatite in order to improve the mechanical properties. Four conditions have been tested to evaluate which are those with better biological properties. Viability and proliferation tests, as well as differentiation assays and morphology observation, are performed with human osteoblast cultures onto the studied coatings. The hydroxyapatite–TiO2 coatings maintain good cell viability and proliferation, especially the cases with higher amorphous phase amount and specific surface, and promote excellent differentiation, with a higher ALP amount for these cases than for polystyrene controls. Observation by SEM corroborates this excellent behaviour. In conclusion, these coatings are a good alternative to those used industrially, and an interesting issue would be improving biological behaviour of the worst cases, which in turn show the better mechanical properties.
Keywords: Bio-ceramic, coating, in vitro behaviour, thermal spray, HVOF
