Toward completely constructed and cellularized blood vessels

Issue title: Selected papers from the 4th China–France Biotherapy and Regenerative Medicine International Symposium, Wuhan, June 2011

Article type: Research Article

Authors: Menu, Patrick^; | Stoltz, Jean-François^; | Kerdjoudj, Halima

Affiliations: Centre National de la Recherche Scientifique, Faculté de Médecine, Nancy Université, Université Henri Poincaré, Vandoeuvre-lès-Nancy, France | CHU de Nancy, Unité de thérapie Cellulaire et Tissulaire, Brabois, Vandoeuvre-lès-Nancy, France | EA Biomatériaux et inflammation en site osseux, BIOS, Faculté de Chirurgie Dentaire, Université Reims Champagne Ardenne, Reims, France

Note: [] Address for correspondence: Patrick Menu, Centre National de la Recherche Scientifique 7561, Faculté de Médecine, Nancy Université, Université Henri Poincaré, 54505 Vandoeuvre-lès-Nancy, France. E-mail: [email protected].

Abstract: Vascular tissue engineering aims to develop implantable blood-vessels, exhibiting biological and biomechanical characteristics close to those of the native vessels. The ultimate goal of our group is to engineer suitable blood vessel substitutes which could be stored for a long time in vascular bank conditions. First attempts tried to develop coating procedures allowing endothelial cells (EC) differentiation, adhesion and retention on current vascular substitutes but the weak in vivo patency of these grafts was related. Since 2003, our group have been evaluated a new surface modification of internal surface of blood vessels based on polyelectrolyte films coating. The layer-by-layer self-assembly and the resulting polyelectrolyte multiplayer films (PEM) is a simple and versatile way to engineer surfaces with highly specific properties. Previous studies indicated that the poly(sodium-4 styrene sulfonate)/poly (allylamine hydrochloride) PSS/PAH multilayered films when ended by PAH, induce strong adhesion and retention of mature EC which spread and keep their phenotype as well on glass, on expanded polytetrafluoroethylene ePTFE and on cryopreserved arteries. The mechanical properties (compliance), leading to early intimal hyperplasia and graft failure, were lost after artery cryopreservation. We have demonstrated that the compliance and elasticity restoration of PEM treated cryopreserved arteries close to native arteries. In other respect, the use of the circulating progenitor which could be differentiated into matures vascular cell offers new opportunities in vascular engineering. Currents protocols, expend at least 1 month to observe both smooth muscle (SMCs) and endothelium (ECs)-like morphology and about two months for confluent monolayer cells. The progenitor cells cultivated on PEM treated glass showed mature and functional vascular cells (SMCs and ECs) development after only 14 days of culture. The morphological appearance, mature and healthy phenotype markers expression and repartition of differentiated cells are close to mature cells. Challenge now is to build up in less a month, an autologous cellularized vascular graft using patient peripheral stem cells.

Keywords: Bioengineering, vascular substitutes, circulating progenitor, polyelectrolyte multiplayer films

DOI: 10.3233/BME-2012-0685

Journal: Bio-Medical Materials and Engineering, vol. 22, no. 1-3, pp. 17-20, 2012