Collagen-based biomaterials and cartilage engineering. Application to osteochondral defects

Issue title: Medical Engineering and Therapy, Nancy 2006, 15–16 May

Article type: Research Article

Authors: Chajra, H.^; | Rousseau, C.F. | Cortial, D. | Ronzière, M.C. | Herbage, D. | Mallein-Gerin, F. | Freyria, A.M.^;

Affiliations: IBCP, Institut de Biologie et Chimie des Protéines, Lyon; CNRS, UMR 5086, University of Lyon; University of Lyon 1; IFR 128, Lyon, France | SYMATESE biomatériaux, ZI Les troques, Chaponost, France

Note: [] Address for correspondence: A.M. Freyria, IBCP, 7 passage du Vercors, 69007 Lyon, F-69367, France. Tel.: +33 (0) 472 722 617; Fax: +33 (0) 472 722 604; E-mail: [email protected].

Abstract: Articular cartilage has a limited capacity for self-repair after trauma. Besides the conventional surgical techniques for repairing such defects, treatments involve implantation of autologous cells in suspension or within a variety of cell carrying scaffolds such as hyaluronic acid, alginate, agarose/alginate, fibrin or collagen. For the repair of full-thickness osteochondral defects, tissue engineers started to design single- or bi-phased scaffold constructs often containing hydroxyapatite-collagen composites, usually used as a bone substitute. The purpose of this study was to compare the behavior of bovine chondrocytes cultured in collagen-based scaffolds containing or not hydroxyapatite and cross-linked following two different methods. Calf chondrocytes seeded within Hemotèse® and Collapat® II sponges (SYMATESE biomaterials), chemically cross-linked with glutaraldehyde or EDC/NHS, were maintained up to one month in culture. The cells exhibited a similar behavior in the four scaffolds regarding proliferation level, deposition of glycosaminoglycans in the scaffolds and gene expression of types I, II and X collagens, aggrecan, MMP-1, -13 and the integrin subunits α10 and α11.

Journal: Bio-Medical Materials and Engineering, vol. 18, no. s1, pp. 33-45, 2008