Affiliations: Cell and Tissue Engineering Department, LEMTA UMR-CNRS 7563, 2 avenue de la forêt de Haye, 54 500 Vandoeuvre, France | INSERM U595, Faculty of Medecine, 67 000 Strasbourg, France | Département de Chimie Physique des réactions, UMR 7630 CNRS-INPL, Groupe ENSIC, 1, rue de Grandville, 54 000 Nancy, France
Note: [] Corresponding author: Dr X. Wang CR/CNRS LEMTA-UMR 7563, 2 avenue de la forêt de Haye 54 500, Vandoeuvre-lès-Nancy, France. Tel.: +33 3 83 59 57 36; Fax: +33 3 83 59 55 44; E-mail: [email protected].
Abstract: Nowadays, synthetic biodegradable polymers, such as aliphatic polyesters, are largely used in tissue engineering. They provide several advantages compared to natural materials which use is limited by immunocompatibility, graft availability, etc. In this work, poly(L-lactic) acid (PLLA), poly(DL-lactic) acid (PDLA), poly-ε-caprolactone (PCL), poly(L-lactic)-co-caprolactone (molar ratio 70/30) (PLCL) were selected because of their common use in tissue engineering. The membranes were elaborated by solvent casting. Membrane morphology was investigated by atomic force microscopy. The membranes were seeded with human fibroblasts from cell line CRL 2703 in order to evaluate the biocompatibility by the Alamar blue test. The roughness of the membranes ranged from 4 nm for PDLA to 120 nm and they presented very smooth surface except for PCL which beside a macroscopic structure due to its hydrophobicity. Human fibroblasts proliferated over 28 days on the membranes proving the non-in vitro toxicity of the materials and of the processing method. A further step will be the fabrication of three-dimensional scaffold for tissue engineering and the treatment of the scaffolds to augment cell adhesion.
