Affiliations: Laboratoire ATOSEP, Université d'Orléans, Rue de Vendôme, BP 6237, 45062 Orléans cedex 2, France | IPROS, Inserm U658, CHR Orléans, 1 rue Porte Madeleine, BP 2439, 45032 Orléans cedex, France
Note: [] Corresponding author: Beaupied Hélène, IPROS, Inserm U658, CHR Orléans, 1 rue Porte Madeleine, BP 2439, 45032 Orléans cedex, France. Tel.: +33 238744067; Fax: +33 238744024; E-mail: [email protected].
Abstract: The bone samples used in clinical and experimental trials must be the less damaged as possible to avoid alterations of their properties. However, the mode of storage might possibly alter the bone properties, particularly microarchitecture and strength. The aim of our study was to analyze the effects of deep-freezing and alcohol conservation techniques on the densitometric, microarchitectural and biomechanical parameters of rat femurs. The left femurs were elongated in uniaxial tension up to breakdown in order to calculate biomechanical parameters. The densitometric and microarchitectural properties of right femurs were evaluated using dual-energy X-ray absorptiometry and microcomputed tomography, respectively. Results showed no significant difference in the parameters investigated between deep-freezing, alcohol storage and fresh femurs when comparing each parameter separately. Therefore, one month storage in alcohol or deep-freezing seemed to induce no harmful effect on densitometric, microarchitectural and biomechanical parameters of rat femurs.
