Use of Enzyme-Linked Immunosorbent Assay (ELISA) for Detection and Quantification of Monoclonal Antibodies

Article type: Research Article

Authors: Lewis, Gladius^;

Affiliations: Department of Mechanical Engineering, The University of Memphis, Memphis, TN 38152, USA

Note: [] Requests for reprints should be addressed to Dr. Gladius Lewis, Department of Mechanical Engineering, The University of Memphis, Memphis, TN 38152, USA.

Abstract: The thrust of the present work is the demonstration of the feasibility of using an enzyme-linked immunosorbent assay (ELISA) for the detection and quantification of a monoclonal antibody human serum. The antibodies used, 7E3 and D3GP3, have been proposed as platelet receptor blockers, being directed specifically against the platelet membrane receptors (glycoprotein IIb/IIIa) and thus of significance in the management of patients at a very high risk of thrombotic occlusion. It is shown that 7E3 is more easily adsorbed than D3GP3 and hence has a higher potential for the stated application.

Keywords: glycoprotein (GP) IIb/IIIa, monoclonal antibodies, enzyme-linked irnrnunosorbent assay (ELISA)

DOI: 10.3233/BME-1994-4503

Journal: Bio-Medical Materials and Engineering, vol. 4, no. 5, pp. 363-367, 1994