Diamond-Like Carbon as Biological Compatible Material for Cell Culture and Medical Application

Article type: Research Article

Authors: Lu, Luo | Jones, Michael W. | Wu, Richard L. C.

Affiliations: Department of Physiology and Biophysics, Wright State University, Dayton, OH 45435, USA | UES Inc., 4401 Dayton-Xenia Road, Dayton, OH 45432, USA

Abstract: Ion beam assisted diamond-like carbon (DLC) films have been used for growing the human hematopoietic myeloblastic ML-1 cells and human embryo kidney 293 cells in the control environment. DLC films were directly deposited onto the P-35 plastic dishes by impacting the high kinetic energy (1000 eV) of methane ions at room temperature. The present results showed that both ML-1 and HEK 293 cells continuously grow with and without DLC films. It has demonstrated that human cells proliferated on DLC film with very high viability and DLC material had no toxicity to cultured human ML-1 and HEK 293 cells. We conclude that DLC film is a biological compatible material for potential cell culture matrix and bio-medical applications.

Keywords: diamond-like carbon, bio-materials, biocompatibility, human cell culture

DOI: 10.3233/BME-1993-3405

Journal: Bio-Medical Materials and Engineering, vol. 3, no. 4, pp. 223-228, 1993