Preparation and osteocompatibility of hydroxyapatite coated on titanium from the reaction of sputtered CaO and vaporized P₂O₅

Article type: Research Article

Authors: Ozeki, K.^; | Yuhta, T. | Aoki, H. | Fukui, Y.

Affiliations: Applied Systems Engineering, Graduate school of Science and Engineering, Tokyo Denki University, Ishizaka, Hatoyama, Hiki, Saitama, 350‐0394, Japan | Frontier Research and Development Center, Tokyo Denki University, Ishizaka, Hatoyama, Hiki, Saitama, 350‐0394, Japan

Note: [] Corresponding author. Tel.: +81 (0)49 296 2911(3752); Fax: +81 (0)49 296 2925; E‐mail: [email protected].

Abstract: Hydroxyapatite (HA) and other calcium phosphates were synthesized on titanium plates by a solid–gas state reaction of sputtered CaO and vaporized P2O5. The calcium phosphates formed were HA, β‐tricalcium phosphate (β‐TCP; Ca3(PO4)2), β‐calcium pyrophosphate (β‐PYR; Ca2P2O7), and β‐calcium metaphosphate (β‐MET; Ca2(PO3)2). Their formation depended on the ratio of the sputtered CaO and the reacting P2O5. For a mole ratio of CaO/P2O5=4 (Ca/P=2), an HA film was synthesized. The surface roughness increased by over seven times after the solid–gas state reaction from Ra = 0.16±0.02 μm (for the CaO film) to Ra = 1.15±0.25 μm (for the reacted film). The synthesized HA film‐coated titanium plates and control non‐coated titanium plates were implanted in the femora of two dogs for a period of two, four and 12 weeks, and observed using a soft X‐ray radiograph and histological sections. New bone formation was observed without any connective tissue at four weeks around the HA film, whereas over the 12 week experimental period, there was no new bone formation around the control and connective tissue was observed over all periods, reaching a thickness of more than 200 μm at 12 weeks.

Keywords: Sputtered CaO, hydroxyapatite, vaporized P[TeX:] $_{2}$O[TeX:] $_{5}$, osteocompatibility

Journal: Bio-Medical Materials and Engineering, vol. 13, no. 1, pp. 83-90, 2003