Affiliations: Orthopeadic Surgery Department of the L. Rydygier's Hospital, os. Złotej Jesieni 1, Cracow, Poland | Laboratory of Experimental Hematology, Institute of Zoology, Jagiellonian University, Ingardena 6, Cracow, Poland
Note: [] Corresponding author: Prof. Zbigniew Dąbrowski, Laboratory of Experimental Hematology, Institute Zoology, Jagiellonian University, ul. Ingardena 6, PL‐30‐060 Kraków, Poland. Tel.: +48 12 633 63 77, ext. 2453; Fax: +48 12 634 07 85; E‐mail: [email protected].
Abstract: In a hematopoietic microenvironment in vivo, spatial organisation of hematopoiesis is possible due to the existence of a three‐dimensional framework, the main part of which is formed by a branching population of stromal cells. Most of the previous in vitro studies, concerning long‐term bone marrow cultures, were based on a previously prepared, flat adherent layer of stromal cells. There are only few reports concerning the three‐dimensional growth pattern of the bone marrow stroma. In the present study we used a new three‐dimensional model of the stromal cell culture. The framework for the cultured stromal cells was a structure of a nonliving trabecular bone (Unilab Surgibone). After a period of about four weeks the stromal cells created a spatial network which filled the intertrabecular spaces of the spongy bone.
