3D printed mesoporous bioactive glass, bioglass 45S5, and β-TCP scaffolds for regenerative medicine: A comparative in vitro study

Article type: Research Article

Authors: Pacheco-Vergara, Maria Jesus^a | Ricci, John L.^a | Mijares, Dindo^a | Bromage, Timothy G.^b | Rabieh, Sasan^b | Coelho, Paulo G.^{c; d} | Witek, Lukasz^{a; e;}

Affiliations: [a] Biomaterials Division, New York University College of Dentistry, New York, NY, USA | [b] Department of Molecular Pathobiology, New York University College of Dentistry, New York, NY, USA | [c] Division of Plastic Surgery, Department of Surgery, University of Miami Miller School of Medicine, Miami, FL, USA | [d] Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, FL, USA | [e] Department of Biomedical Engineering, New York University Tandon School of Engineering, Brooklyn, NY, USA

Correspondence: [*] Corresponding author: Lukasz Witek, Biomaterials Division, New York University College of Dentistry, 345 E. 24th St., Room 902D, New York 10010, NY, USA. E-mail: [email protected]

Abstract: BACKGROUND:While autografts to date remain the “gold standard” for bone void fillers, synthetic bone grafts have garnered attention due to their favorable advantages such as ability to be tailored in terms of their physical and chemical properties. Bioactive glass (BG), an inorganic material, has the capacity to form a strong bond with bone by forming a bone-like apatite surface, enhancing osteogenesis. Coupled with additive manufacturing (3D printing) it is possible to maximize bone regenerative properties of the BG. OBJECTIVE:The objective of this study was to synthesize and characterize 3D printed mesoporous bioactive glass (MBG), BG 45S5, and compare to β-Tricalcium phosphate (β-TCP) based scaffolds; test cell viability and osteogenic differentiation on human osteoprogenitor cells in vitro. METHODS:MBG, BG 45S5, and β-TCP were fabricated into colloidal gel suspensions, tested with a rheometer, and manufactured into scaffolds using a 3D direct-write micro-printer. The materials were characterized in terms of microstructure and composition with Thermogravimetric Analyzer/Differential Scanning Calorimeter (TGA/DSC), Fourier Transform Infrared Spectroscopy (FTIR), X-ray Diffraction (XRD), Micro-Computed Tomography (μ-CT), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDS), and Mattauch–Herzog-Inductively Coupled Plasma-Mass Spectrometry (MH-ICP-MS). RESULTS:Scaffolds were tested for cell proliferation and osteogenic differentiation using human osteoprogenitor cells. Osteogenic media was used for differentiation, and immunocytochemistry for osteogenic markers Runx-2, Collagen-I, and Osteocalcin. The cell viability results after 7 days of culture yielded significantly higher (p < 0.05) results in β-TCP scaffolds compared to BG 45S5 and MBG groups. CONCLUSION:All materials expressed osteogenic markers after 21 days of culture in expansion and osteogenic media.

Keywords: 3D printing, bioglass 45S5, mesoporous bioactive glass, β-TCP, scaffolds, in vitro,osteogenic differentiation

DOI: 10.3233/BME-222524

Journal: Bio-Medical Materials and Engineering, vol. 34, no. 5, pp. 439-458, 2023