Attachment and conformational changes of collagen on bioactive glass surface

Article type: Research Article

Authors: Magyari, K. | Vanea, E. | Baia, L. | Simon, V.^{; *}

Affiliations: Faculty of Physics & Interdisciplinary Research Institute on Bio-Nano-Sciences, Babes-Bolyai University, 400084 Cluj-Napoca, Romania

Correspondence: [*] Corresponding author. Tel.: +40 264 405300; Fax: +40 264 591906; E-mail: [email protected].

Abstract: The proteins adsorption on biomaterials surface leads to changes in their structural conformation that may further influence the adhesion, migration and growth of cells. The aim of this study was to examine the attachment of collagen (calf skin type I) on bioactive glass powders and the conformational changes of the protein. Scanning electron microscopy analysis and X-ray photoelectron spectroscopy measurements indicate that the collagen cover the glass surface in a nanometric thin layer. The infrared amide I absorption signal shows pronounced changes in the secondary structure of the adsorbed collagen.

Keywords: Collagen, adsorption, SEM, XPS, FT-IR

DOI: 10.3233/BME-161567

Journal: Bio-Medical Materials and Engineering, vol. 27, no. 1, pp. 63-74, 2016