Affiliations: [a] Department of Engineering of Materials and Bioprocesses, School of Chemical Engineering, University of Campinas, Campinas-SP, Brazil | [b] Department of Applied Physics, Institute of Physics “Gleb Wataghin”, University of Campinas, Campinas-SP, Brazil
Correspondence:
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Corresponding author: Andréa Arruda Martins Shimojo, Department of Engineering of Materials and Bioprocesses, School of Chemical Engineering, University of Campinas, Campinas-SP, Brazil. E-mail: [email protected].
Abstract: This work evaluated the effects of UV irradiation, plasma radiation, steam and 70% ethanol treatments on the sterilization and integrity of auto-crosslinked hyaluronic acid (HA-ACP) scaffolds structured in microparticles and sponges aiming in vivo applications for regenerative medicine of bone tissue. The integrity of the microparticles was characterized by rheological behavior, while for the sponges, it was characterized by scanning electron microscopy, Fourier transform infrared spectroscopy and differential scanning calorimetry. The effectiveness of the sterilization treatment was verified by the number of microorganism colonies in the samples after the treatments. In conclusion, plasma radiation was the best treatment for the sponges, while steam sterilization in the autoclave at 126°C (1.5 kgf/cm2) for 5 min was the best treatment for the microparticles.
