Metal patch test is often used in clinical settings when metal-induced contact dermatitis is suspected. However, the transdermal permeation behavior of metal ions from the patch test remains unclear. Current patch tests using high concentrations of metal salt solutions have some side effects, e.g. acute skin reactions to high concentrations of metal salt. To resolve these, estimating metal ion transdermal permeation is wished. In this study, synchrotron radiation X-ray fluorescence (SR-XRF) and micro-focused particle-induced X-ray emission (micro-PIXE) were used to visualize the time-dependent Ni permeation in mouse skin. The cross-sectional diffusion of Ni was visualized in a time-dependent manner. Our results indicate that maximum Ni permeation occurs after 24 h of patch treatment, and the permeated Ni content was high in the epidermis and spread into the dermis beyond the basal layer. This method may be useful to determine the appropriate solution concentration and duration of administration for the patch test.
Metal allergy patch tests are widely used to diagnose metal-induced contact dermatitis [1,2]. Current patch tests use high concentrations of metal in aqueous salt solutions and a reservoir sheet that allows the test solution to permeate into the skin and induce a local allergic reaction. Although this test is routinely performed to diagnose metal allergies, false-positive and negative reactions are frequently observed [3,4]. Acute high doses of metal ions can directly damage skin tissue, thus preventing accurate allergy diagnosis. For example, Fischer et al. reported that skin irritation and false positives can occur when using the patch test with high concentrations (5% w/v) of Ni sulfate . Thus, side effects can be avoided by using the minimum concentration of metal salt solution required to induce a local allergic reaction. To achieve this, a method to estimate metal ion transdermal permeation kinetics is essential. The total Ni ion permeation  and the permeation depth have been reported using a tape stripping method [7,8]. In those reports, an adhesive tape was applied to the Ni permeated skin under constant pressure and gradually removed. This process was repeated 20 times and the Ni concentration in each peeled stratum corneum was evaluated using inductively coupled plasma mass spectroscopy (ICP-MS). Each tape strips removed one layer of corneocytes, approximately. Then, the depth profile of permeated Ni in the skin layer was estimated. However, visualization of the cross-sectional distribution of permeated Ni ions with various patch application periods would allow for a better understanding of permeation behavior. We have applied synchrotron radiation X-ray fluorescence (SR-XRF), and micro-focused particle-induced X-ray emission (micro-PIXE) for the distribution imaging of the trace elements contained in human soft tissues [9–11]. SR-XRF method irradiates the monochromatized synchrotron radiation X-rays to the specimens and the generated characteristic (fluorescence) X-rays are detected. SR-XRF spectrum shows fluorescence X-ray peaks derived from various elements contained in the specimen with a negligible background. Therefore, weak fluorescence X-ray peaks derived from trace elements could be easily detected . X-ray irradiation seldom damages specimens, then, SR-XRF is suitable for the non-destructive analysis of trace elements in the biomedical specimens. SR-XRF with micro-focused X-rays provides the micro-area analysis and elemental distribution analysis. Micro-PIXE method irradiates the focused proton beams to the specimen and generated characteristic X-rays are detected. The specimen damage by the proton irradiation was quite small, then, the non-destructive analysis also could be carried. The focul size of the proton beams are smaller than the typical focused X-ray used in SR-XRF. Therefore, detailed elemental distribution can be obtained by micro-PIXE. In this study, we applied those highly sensitive elemental analysis method to visualize the time-dependent cross-sectional distribution of Ni using a patch test applied to the epidermis.
2.Materials and methods
C57BL/6 mice (50 weeks old) were used in this study. All animal protocols were approved by the Animal Ethics Review Board of the Dental Hospital of Nihon University School of Dentistry, Tokyo, Japan, and conformed to the guidelines of the National Institutes of Health. The back skin of all mice was depilated under general anesthesia. The test solution (5% w/v NiSO4 aq.) and closed patch test sheet (Torii Pharmaceutical, Tokyo, Japan) were applied to the depilated skin. The application period was varied between 30 min and 48 h, after which the mice were slaughtered. The patch was removed and the back skin was gently wiped. The skin that contacted the patch test was excised and immediately frozen in crushed dry ice. All specimens were cut into 1-cm-long sections at −24°C. The lower part of the skin specimen was embedded in optimum cutting temperature (OCT) compound (Sakura Finetek, Tokyo, Japan) on the cryostat base, and the upper part of the specimen was cut into sections (20 µm) using a cryostat (Leica Microsystems, Wetzlar, Germany). The sectioned specimens were placed on Kapton film (12.5 µm; Du Pont-Toray, Tokyo, Japan), dried, and subjected to elemental distribution analyses. Adjacent specimen slices were subjected to hematoxylin and eosin (H–E) staining for histopathological analysis.
Elemental distribution was analyzed by two different methods, depending on the observation area and resolution. Analysis of the entire specimen was performed using SR-XRF. The micro-focused incident X-ray (12.9 KeV) was irradiated, and the specimen stage was two-dimensionally step-scanned. The fluorescence X-ray was detected at each point, and the elemental distribution images were processed. The high-resolution elemental distribution was analyzed using micro-PIXE analysis, which was performed under irradiation by application of a micro-focused proton beam with raster scanning over the target area of the specimen. The characteristic X-rays were detected, and the elemental distribution image was processed. Detailed conditions of the analyses are shown in Table 1.
|SR-XRF analysis||Facility||BL-4A (Photon Factory, High Energy Accelerator Research Organization, Tsukuba, Japan)|
|The electron storage ring was operated at 2.5 GeV and 450 mA.|
|Incident X-ray energy = 12.9 KeV|
|Focal size = 20 µm using polycapillary optics|
|Measurement step = 40 µm (1 sec steps)|
|Data process||PyMca (ESRF, Ver 4.7.3)|
|Micro-PIXE analysis||Facility||PASTA (National Institute of Radiological Sciences, Chiba, Japan)|
|Micro-focused proton beam (3.0 MeV, 2 µm beam diameter, total irradiation = 200 nC)|
|Data process||OMDAQ2007 (Oxford Microbeams Ltd., Version 220.127.116.119)|
Figure 1 shows the H–E stained histopathological images and the elemental distribution images by SR-XRF from the skin cross sections treated with patches for 24 h. The S distribution image shows the shape of the specimens. The permeated Ni from the patch was clearly observed in the surface of the skin. The histological images and the Ni distribution images by SR-XRF of the specimens after 30 min to 48 h of patch exposure are shown in Fig. 2. All SR-XRF measurements were taken under the same ring current and optical geometry. The fluorescent X-ray intensity from each specimen was comparable, as was the relative content. Figure 3 shows the relative content of Ni in the skin over time as evaluated by the integrated fluorescence X-ray intensity of Ni Kα over the entire specimen area (as shown in Fig. 2). The Ni relative content increased in a time-dependent manner, reaching a maximum at 24 h post-application. The Ni relative content decreased after 48 h.
The detailed elemental distribution images (S, P and Ni) from the areas with high Ni accumulation (black and white squares in Fig. 2), as assessed by micro-PIXE, are shown in Fig. 4. Additionally, H–E stained images from the same area are also shown. Ni permeation was clearly observed 30 min after patch application. Ni content in skin, which corresponded to the characteristic X-ray intensity, was increased in a time-dependent manner, reaching a maximum at 24 h post-application. Ni content subsequently decreased after 48 h. The permeated Ni content was high in the epidermis, and spread into the dermis beyond the basal layer. The decrease in Ni content after 48 h is like due to complete permeation of Ni from the patch test sheet and diffusion and intravascular invasion of the permeated Ni from the skin. Permeation of high concentrations of Ni into the skin was reported to increase blood flow [12,13]. Thus, the removal of Ni by blood flow may cause the decreased Ni concentration after 48 h. An in vitro study evaluating Ni permeation through the human stratum corneum also reported that the permeation rate of aqueous Ni solutions was highest around 24 h, regardless of the Ni salt species [3,4], in agreement with our results. Current metal allergy tests apply patches for 48 h and diagnose allergies 48 h to 1 week after patch application. According to Figs 3 and 4, the 48 h application period is sufficient to permeate the Ni ion; however, most Ni permeation is completed after 24 h. Thus, the patch application period could be shortened. Localization of P was observed in the same areas as Ni. As shown by the histopathological analysis, P is derived from inflammatory cells. Thus, the co-localization of Ni and P suggests that Ni permeation from the patch induces local inflammation.
In the present study, we applied SR-XRF and micro-PIXE to visualize permeated Ni by using a Ni allergy patch test applied to mouse skin. The skin specimens were freeze-sectioned, and the cross-sectional Ni distribution was clearly visualized using two different techniques. The distribution of Ni in the entire specimen could be observed using SR-XRF with lower resolution, and a high-resolution image could be obtained using micro-PIXE within a restricted region of interest in the same specimen. In addition, H–E imaging of the adjacent section provided histopathological information. Thus, the feasibility of these analytical methods to visualize metal ion permeation behavior from the metal allergy patch test was demonstrated.
The direct observation method used to assess metal permeation during the patch test revealed the time-dependent permeation and cross-sectional distribution of metal ions. This information would be useful to estimate the optimum concentration for applied metal salt solutions and the application period for the metal allergy patch test. Concerning to the permeation difference of metal ion species, Kubo et al. reported different behavior when using Cr ions in mouse tails . This study showed slow permeation, with the lower layer of the stratum corneum serving as a barrier. While, Ni was permeated into the dermis beyond the basal layer in this study. Thus, differences in metal ion charge and species may affect mobility. The present method is applicable for all metal ions; thus, the metal ion permeation behavior using various patch tests could be estimated and compared. Quantitative analysis should be carried out; however, a method of preparation using low concentration standards has not been established. The quantification of permeated metal ions, including both Ni and other species, should be carried in future.
SR-XRF and micro-PIXE were used to analyse Ni allergy patch application in mouse skin. Capillary focused SR-XRF is appropriate to obtain a rapid, wide area scan with low resolution. Micro-PIXE allows for a high resolution analysis within a restricted region of interest. These methods are both non-destructive. As a result, the direct observation of the permeated metal ions during the patch test could be carried out using these methods in combination. The time-dependent permeation and cross-sectional distribution of metal ions were revealed. This information would be useful to estimate the metal ion permeation of the metal allergy patch test.
The SR-XRF measurements were performed with the approval of the Photon Factory Program Advisory Committee (Proposal No. 2014G017). This work was financially supported by the Japan Society for the Promotion of Science KAKENHI (grant No. 23390438 to M. Uo). T. Sugiyama is a supported by a research fellowship for young scientists from the Japan Society for the Promotion of Science.
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