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Development of SPR biosensor for simultaneous detection of multiplex respiratory viruses

Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 4th International Conference on Biomedical Engineering and Biotechnology, 18–21 August 2015, Shanghai, China

Guest editors: Feng Liu, Dong-Hoon Lee, Ricardo Lagoa and Sandeep Kumar

Article type: Research Article

Authors: Shi, Leia; b | Sun, Qiuxiangc | He, Jian’and | Xu, Huad | Liu, Chunxiaod | Zhao, Chunzhongd | Xu, Yunqingd | Wu, Changlind | Xiang, Junjiana | Gu, Dayongd | Long, June | Lan, Hekuie; *

Affiliations: [a] Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Antibody Engineering Center of Jinan University, Guangzhou, 510632, P.R. China | [b] Shenzhen Academy of Inspection and Quarantine, Shenzhen, 518010, P.R. China | [c] Feng Gang Hospital, Dongguan, 523695, P.R. China | [d] Central Laboratory of Health Quarantine, Shenzhen International Travel Health Care Center, Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen, 518033, P.R. China | [e] Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, P.R. China

Correspondence: [*] Address for correspondence: Hekui Lan, Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou, 510282, P.R. China. Tel.: 086-020-62782999; Fax: 086-0755-83394162; E-mail: [email protected].

Note: [1] Lei Shi and Qiuxiang Sun contributed equally to this work.

Keywords: SPR, respiratory virus, high throughput, label-free, gene chip

DOI: 10.3233/BME-151526

Journal: Bio-Medical Materials and Engineering, vol. 26, no. s1, pp. S2207-S2216, 2015

Published: 2015
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A surface plasmon resonance (SPR)-based biosensor was developed for specific detection of nine common respiratory virus, including influenza A and influenza B, H1N1, respiratory syncytial virus (RSV), parainfluenza virus 1-3 (PIV1, 2, 3), adenovirus, and severe acute respiratory syndrome coronavirus (SARS). The SPR biosensor was developed by immobilizing nine respiratory virus-specific oligonucleotides in an SPR chip. To increase the biosensor sensitivity, biotin was used to label the PCR primer and further amplify the signal by introducing streptavidin after hybridization. Throat swab specimens representing nine common respiratory viruses were tested by the innovative SPR-based biosensor to evaluate the sensitivity, specificity and reproducibility of this method. Results suggest that this biosensor has the potential to simultaneously identify common respiratory viruses.

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