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Abnormal increased re-released Hb from RBCs of an intrahepatic bile duct carcinoma patient was detected by electrophoresis release test

Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 4th International Conference on Biomedical Engineering and Biotechnology, 18–21 August 2015, Shanghai, China

Guest editors: Feng Liu, Dong-Hoon Lee, Ricardo Lagoa and Sandeep Kumar

Article type: Research Article

Authors: Su, Yana | Han, Lishaa | Gao, Lijuna | Guo, Junb | Sun, Xiaoronga | Li, Jiaxina | Qin, Wenbina; *

Affiliations: [a] Laboratory of Hemoglobin, Baotou Medical College, Baotou, 014060, China | [b] Department of Invasive Technology, the Seventh Hospital of Baotou, Baotou, 014010, China

Correspondence: [*] Address for Correspondence: Wenbin Qin, Laboratory of Hemoglobin, Baotou Medical College, 31# Jianshe Road, Donghe District, Baotou, Inner Mongolia, 014060, China. Tel.: 86-472-7167834; Fax: 86-472-7167834; E-mail: [email protected].

Note: [1] Yan Su and Lisha Han contributed to this paper equally.

Keywords: Hemoglobin, red blood cell, electrophoresis release test, intrahepatic bile duct carcinoma

DOI: 10.3233/BME-151510

Journal: Bio-Medical Materials and Engineering, vol. 26, no. s1, pp. S2049-S2054, 2015

Published: 2015
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In this paper, the hemoglobin (Hb) re-released from red blood cells (RBCs) and whole blood of 7 carcinoma patients were studied by using electrophoresis release test (ERT), which was established by our lab. Among the 7 carcinoma patients, the re-released Hb was distinctively increased from an intrahepatic bile duct carcinoma patient during one-dimension isotonic ERT. Different from the others, the result of double-dimension Hb re-release of this intrahepatic bile duct carcinoma patient showed that not only HbA but also HbA2 could be re-released from both RBCs and whole blood. The result of isotonic & hypotonic ERT which was performed at room temperature showed that more Hb could be re-released from both RBCs and whole blood of the intrahepatic bile duct carcinoma patient than that of the normal control. After keeping the samples at 37°C for 1 hour, the re-released Hb from RBCs could still be found more than that of the normal control, but was disappeared completely from the whole blood sample. To our surprise, when the isotonic & hypotonic ERT was repeated 2 days later at 37°C, the re-released Hb from RBCs of the intrahepatic bile duct carcinoma patient was increased only in tube 4-6, and disappeared in the other tube. Further mechanism research work cannot be continued because of the patient’s leave, but ERT is speculated to be a useful and effective technology to observe the physiological or pathological change of RBCs, blood or body in the future.

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