You are viewing a javascript disabled version of the site. Please enable Javascript for this site to function properly.
Go to headerGo to navigationGo to searchGo to contentsGo to footer
In content section. Select this link to jump to navigation

The effect of different molecular weight collagen peptides on MC3T3-E1 cells differentiation

Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 4th International Conference on Biomedical Engineering and Biotechnology, 18–21 August 2015, Shanghai, China

Guest editors: Feng Liu, Dong-Hoon Lee, Ricardo Lagoa and Sandeep Kumar

Article type: Research Article

Authors: Liu, Junlia; b | Si, Shaoyanb | Qin, Yayab | Zhang, Bina | Song, Shujunb; * | Guo, Yanchuana; *

Affiliations: [a] Key Laboratory of Photochemical Conversion and Functional Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, People’s Republic of China | [b] Special Disease Medical Research Center, People’s Liberation Army 306 Hospital, Beijing, People’s Republic of China

Correspondence: [*] Address for correspondence: Shujun Song, Special Disease Medical Research Center, People’s Liberation Army 306 Hospital, Beijing, 100101, People’s Republic of China. Tel.: +86-10-66354547; Fax: +86-10-66356306; E-mail: [email protected]. Yanchuan Guo, Key Laboratory of Photochemical Conversion and Functional Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing, 100190, People’s Republic of China. Tel.: +86-10-82543583; Fax: +86-10-62554670; E-mail: [email protected].

Keywords: Different molecular weight collagen peptides, MC3T3-E1 cells, differentiation

DOI: 10.3233/BME-151509

Journal: Bio-Medical Materials and Engineering, vol. 26, no. s1, pp. S2041-S2047, 2015

Published: 2015
Get PDF open access

Our study aimed at investigating the effect of different molecular weight bovine collagen peptides, namely CH878, CH1370, CH2900, and CH7747 on the differentiation of MC3T3-E1 cells. Osteogenic differentiation of MC3T3-E1 cells was assessed by a series of specific assays, after culturing of cells in the presence of collagen peptides. Alkaline phosphatase activity (ALP) was evaluated by NBT/BCIP staining. Osteocalcin expression was determined by a radioimmunology method. Mineralization was quantified by Alizarin Red staining. ALP staining results demonstrated that the ALP staining of cells after culture in the presence of collagen peptides were significantly higher than the control group (P<0.05), indicating the promotion of ALP activity in MC3T3-E1 cells by these peptides. Radioimmunology results demonstrated that collagen peptides groups were all significantly higher than the control group (P<0.01). Alizarin Red staining results demonstrated that CH1370, CH2900, and CH7747 significantly promoted the formation of mineralized bone matrix. We therefore conclude that CH1370, CH2900, and CH7747 play an active role in the differentiation of MC3T3-E1 cells. Based on the above results, we provide molecular basis for further development of collagens with different molecular weight for the prevention and treatment of osteoporosis.

Show more