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Effect of surface roughness on the initial response of MC3T3-E1 cells cultured on polished titanium alloy

Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 4th International Conference on Biomedical Engineering and Biotechnology, 18–21 August 2015, Shanghai, China

Guest editors: Feng Liu, Dong-Hoon Lee, Ricardo Lagoa and Sandeep Kumar

Article type: Research Article

Authors: Wu, Chunyaa; * | Chen, Mingjuna | Zheng, Tingb | Yang, Xiaonanc

Affiliations: [a] State Key Laboratory of Robotics and System, Harbin Institute of Technology, Harbin, Heilongjiang 150001, China | [b] School of Mechatronics Engineering, Harbin Institute of Technology, Harbin, Heilongjiang 150001, China | [c] State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, Heilongjiang 150090, China

Correspondence: [*] Address for correspondence: Chunya Wu, State Key Laboratory of Robotics and System, Harbin Institute of Technology, Harbin, Heilongjiang 150001, China. Tel.: +86 451 86403252; Fax: +86 451 86403252; E-mail: [email protected].

Keywords: Cell morphology, cell proliferation, surface topography, contact guidance

DOI: 10.3233/BME-151301

Journal: Bio-Medical Materials and Engineering, vol. 26, no. s1, pp. S155-S164, 2015

Published: 2015
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Surface roughness has been considered as an important influencing factor for cell response. The aim of this study was to find out whether MC3T3-E1 cells, a mouse osteoblast-like cell line, can sense the amplitudes of surface topography of titanium alloy (Ti6Al4V), and if surface-dependent cell morphology would be presented on the substrata with varied roughness. A series of polished samples (Ra: 0.30~1.80 μm) were prepared to produce macroscopically parallel grooves using different grades of silicon carbide sandpaper (#100, #320, #600, #1000 and #2000). The experimental results indicated that the behavior and morphology of cells largely depended on the substrata where they were cultured. More efficient proliferation of MC3T3-E1 cells was shown on the surfaces with Ra of 0.50~1.00 μm, with respect to either the rougher or the smoother specimens. Furthermore, MC3T3-E1 cells seeded on the Ti6Al4V surfaces within this narrow range responded to the increasing surface roughness with increased proliferation. Contact guidance of cells could be observed on the rougher specimens (Ra: 0.80~1.00 μm), whereas more random orientations were exhibited for the adsorbed cells on the smoother surfaces (Ra: 0.50~0.60 μm).

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