Construction of biocompatible porous tissue scaffold from the decellularized umbilical artery
Issue title: Selected Papers from the 5th China–France International Symposium “Stem Cells: From Bench to Bedside”, 12–14 December 2013, Kunming, China
Affiliations: Capital Medical University Affiliated Beijing Anzhen Hospital, Beijng Heart Lung and Vessels Disease Research Institute, Beijing, China | The Key Laboratory of Remodeling-Related Cardiovascular Diseases, Capital Medical University, Ministry of Education, Beijing, China | Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Beijing, China | CHU, Unité de Thérapie Cellulaire, Vandœuvre-lès-Nancy, France | CNRS – UMR UL 7365 IMoPA, Faculté de Médecine, Vandœuvre-lès-Nancy, France
Note: [] These authors contribute equally to this work.
Note: [] Address for correspondence: Na Li, Capital Medical University Affiliated Beijing Anzhen Hospital, Beijng Heart Lung and Vessels Disease Research Institute, 2, Anzhenlu, Chaoyang district, Beijing 100029, China. Tel.: +86 10 6445 6509; Fax: +81 10 6445 6095; E-mail: [email protected]
Abstract: The scaffolds prepared from the tissue decellularization conserve the porous 3-D structure and provide an optimal matrix for the tissue regeneration. Since decade, the enzymatic digestion, chemical reagent treatment and mechanical actions such as eversion and abrasion have been used to remove the cells from the intact matrix. In this study, we optimized an enzymatic method to decellularize the umbilical artery to construct a 3-D porous scaffold which is suitable for the culture of mesenchymal stem cells (MSCs). The scaffold maintained the interconnected porous structure. It remained the similar high water content 95.3±1% compared to 94.9±0.6% in the intact umbilical artery (p>0.05). The decellularization process decreased the stress from 0.24±0.05 mPa to 0.15±0.06 mPa (p<0.05). However the decellularization did not change the strain of the artery (45±15% vs. 53±10%, p>0.05). When the scaffold was transplanted to the subcutaneous tissue in the wild type mice, there were less T cells appeared in the surrounding tissue which meant the decreased the immunogenicity by decellularization. This scaffold also supported the adhesion and proliferation of the MSCs. In this study, we constructed a biological compatible porous scaffold from the decellularized umbilical artery which may provide a suitable scaffold for cell-matrix interaction studies and for tissue engineering.
