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Adenoviral-mediated GDNF protects bone marrow mesenchymal stem cells against apoptosis induced by hydrogen peroxide

Issue title: Frontiers in Biomedical Engineering and Biotechnology – Proceedings of the 3rd International Conference on Biomedical Engineering and Biotechnology, 25–28 September 2014, Beijing, China

Article type: Research Article

Authors: Gao, Xiaoqing | Du, Jie | Fan, Guangbi | Yang, Chaoxian | Deng, Li | Yuan, Qionglan;

Affiliations: Department of Anatomy and Neurobiology, Luzhou Medical College, Sichuan Luzhou 646000, China | Department of Anatomy and Neurobiology, Tongji University School of Medicine, Shanghai 200092, China

Note: [] Corresponding author: Qionglan Yuan, Department of Anatomy and Neurobiology, Tongji University School of Medicine, Shanghai 200092, China. Tel.: 021-65985626; Fax: 021-69584636; E-mail: [email protected].

Keywords: Glial cell line-derived neurotrophic factor, bone marrow mesenchymal stem cells, hydrogen peroxide, apoptosis

DOI: 10.3233/BME-141028

Journal: Bio-Medical Materials and Engineering, vol. 24, no. 6, pp. 2169-2176, 2014

Published: 2014
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Abstract

In this study, the effects of adenoviral-mediated glial cell line-derived neurotrophic factor (Ad-GDNF) on apoptosis of cultured bone marrow mesenchymal stem cells (BMSCs) induced by hydrogen peroxide (H2O2) were investigated. After BMSCs infected with Ad-GDNF were treated with 500 μM H2O2 at 37° C for 1 h, lactate dehydrogenase (LDH), MTT and TUNEL methods were used to detect cell viability and apoptosis. In addition, the levels of GDNF in the supernatants in BMSCs cultures were detected by ELISA, pro-inflammatory cytokines IL-6 and TNF-a in the supernatants and apoptosis-related protein Bax and Bcl-2 in cell pellets were investigated. The results showed that H2O2 treatment significantly induced apoptosis of BMSCs and decreased the viability of BMSCs. However, Ad-GDNF significantly reversed the effects of H2O2 on BMSCs. Furthermore, Ad-GDNF significantly decreased the levels of IL-6 and TNF-a and increased Bcl-2/Bax ratio in BMSCs treated by H2O2. In conclusion, Ad-GDNF inhibits apoptosis of BMSCs induced by H2O2, and the mechanisms may be related to down-regulating the expression of IL-6 and TNF-a and up-regulating the ratio of Bcl-2/Bax in BMSCs.

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