Note: [] Corresponding author: Dr. Tamas Alexy, Department of Physiology and Biophysics, Keck School of Medicine, 1333 San Pablo Street, Los Angeles, CA 90033, USA. Tel.: +1 323 442 1268; Fax: +1 323 442 2283; E-mail: [email protected].
Abstract: Cyclic oligomers of glucose, termed cyclodextrins (CDs), can contain 6 (α-CD), 7 (β-CD) or 8 (γ-CD) glucose units and are able to remove cholesterol from platelet membranes and decrease platelet aggregation. The present study was designed to examine the effects of these CDs on RBC aggregation and blood viscosity. Blood from normal adult volunteers was incubated at 37°C with 3.0×10−4 to 1.5 mM levels of the CDs, then processed to obtain platelet-rich plasma, platelet poor plasma and 40% hematocrit blood; measurements included collagen-induced platelet aggregation, RBC aggregation (Myrenne Aggregometer) and blood viscosity at 1–1000 sec−1(Rheolog®). Our results indicate the expected dose-dependent inhibition of platelet aggregation by β-CD, with no significant effects of α-CD or γ-CD. RBC aggregation studies showed no effect of α-CD but highly significant (p<0.01) decreases by both β-CD and γ-CD; at the concentrations studied (1.5×10−3 to 1.5 mM), β-CD had somewhat greater effects. Blood viscosity was not affected by α-CD, but was significantly decreased in a dose-dependent manner by β-CD and, at the highest concentration (1.5 mM), by γ-CD. Interestingly, the effects of β-CD and γ-CD were independent of shear, with these effects not explained by the usual mechanisms. These results suggest the potential hemorheological value of CDs, yet also indicate the need for additional studies.
Keywords: Cyclodextrins, hemorheology, red blood cell, aggregation, platelets
