Affiliations: Dipartimento di Medicina Interna, Malattie Cardiovascolari e Nefrourologiche, Università di Palermo, Italy | Dipartimento di Scienze e Tecnologie Biomediche, Università di L'Aquila, Italy
Note: [] Corresponding author: Prof. Gregorio Caimi, Via Leonardo da Vinci, 52, 90145 Palermo, Italy. Tel.: +39 91 6554406; Fax: +39 91 6554535; E-mail: [email protected].
Abstract: In a group of 18 subjects with acute deep venous thrombosis (DVT), evidenced by clinical examination and echo-color-Doppler, we examined the phenotypical expression of the polymorphonuclear leukocyte (PMN) beta2-integrins (CD11a, CD11b, CD11c, CD18), obtained by using a flow cytofluorimeter. The evaluation was performed before and after in vitro activation (prolonged for 5 and 15 minutes) with 4-phorbol 12-myristate 13-acetate (PMA) and N-formyl-methionyl-leucyl-phenylalanine (fMLP). In DVT subjects, at baseline, the phenotypical expression of CD11b was decreased and that of CD11c was increased when compared with normal controls; no difference was found in CD11a and CD18 expression. In normal subjects PMN activation with both activators led to a constant increase of all PMN adhesion molecules; in DVT subjects CD11b, CD11c and CD18 increased, while CD11a expression did not show any change. These data indicate the presence of a functional alteration in circulating PMN cells from patients with DVT.
Keywords: Deep venous thrombosis, polymorphonuclear leukocyte integrins, polymorphonuclear leukocyte activation
