Adhesion of monocyte‐derived dendritic cells to human umbilical vein endothelial cells in flow field decreases upon maturation

Article type: Research Article

Authors: Jiang, Yuhui | Zeng, Zhu | Sun, Dagong | Ka, Weibo | Wen, Zongyao^;

Affiliations: Department of Biophysics, Peking University Health Science Center, Beijing, China | Department of Medical Physics, Peking University Health Science Center, Beijing, China

Note: [] Corresponding author: Prof. Zongyao Wen, Department of Biophysics, Basic Medical School, Peking University, Beijing 100083, P.R. China. Tel.: +86 10 82802419; Fax: +86 10 62015582; E‐mail: [email protected].

Abstract: Dendritic cells (DC) are sentinels of the immune system. They and their precursors undergo complex migration to perform their function in vivo. Binding of DC to vascular endothelial cells in a flow field has not been investigated. We therefore determined adhesion of DC and their precursors, MO, to human umbilical vein endothelial cells (HUVECs) under various shear stresses by using a flow chamber system. The results showed that the binding was reduced with developmental stages of DC, which partially depended on CD11a and cell surface charges. The data had potential relevance for anti‐cancer immunotherapy strategies favoring the application of mDC.

Keywords: Dendritic cell, vascular endothelial cell, adhesion molecule, shear stress, surface charge

Journal: Clinical Hemorheology and Microcirculation, vol. 32, no. 4, pp. 261-268, 2005