Hemorheological investigation of clotting by rotational viscometer

Article type: Research Article

Authors: Liao, Fu-long | Li, Wen | Huang, Shen

Affiliations: Department of Pharmacology, Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing, China

Note: [] Accepted by: Editor H. Hartert

Abstract: HAAKE Rotovisco RV100/CV100 was employed to investigate coagulation of whole blood, platelet rich plasma (PRP) and plasma. Samples were anticoagulated with sodium citrate. Clotting was initiated by adding CaCl2 Shear stress versus time at shear rate 0.3 s−1 at 37°C was recorded. Two parameters were established: (1) Recalcification time tr; and (2) viscosity increasing rate dη/dt. The parameters appeared to be meaningful to the clotting behaviour of blood, PRP and plasma. Several antithrombotics were tested by the method. Warfarin prolonged tr with a dramatic suppression of dη/dt (P<0.001). Aspirin decreased dη/dt (P<0.001) without significant influence on tr. Heparin showed dose-response correlations for both the parameters. The unconventional test of clotting by rotational viscometer may have its potential in clinical practice of hemorheology.

Keywords: Hemorheology, Blood viscosity, Clotting, Antithrombotics, Aspirin, Heparin

DOI: 10.3233/CH-1987-7502

Journal: Clinical Hemorheology and Microcirculation, vol. 7, no. 5, pp. 671-677, 1987