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Haemorheological changes during human erythrocyte life-span

Article type: Other

Authors: Micheli, V.a | Pieragalli, D.b | Toti, P.c | Forconi, S.b | Guerrini, M.b | Acciavatti, A.b | Galigani, C.b | Weber, G.c | Ricci, C.a | DiPerri, T.b

Affiliations: [a] Istituto di Chimica Biologica, University of Siena, Siena, Italy | [b] Istituto di Patologia Special Medica, University of Siena, Siena, Italy | [c] Istituto di Anatomia Patologia, University of Siena, Siena, Italy

Note: [] Accepted by: Editor T. DiPerri

Keywords: Erythrocyte life-span, Percoll, G6PD, PK, erythocyte filterability, washed red blood cell filterability

DOI: 10.3233/CH-1987-7211

Journal: Clinical Hemorheology and Microcirculation, vol. 7, no. 2, pp. 267-272, 1987

Received 1 December 1986
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Accepted 19 January 1987
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Published: 9 December 2016
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Abstract

The investigation of erythrocyte deformability changes during cell ageing might give one insight on circulatory behaviour of young and old red blood cells.

Erythrocytes of healthy subjects of both sexes had been separated into 3 fractions of increasing age on a Percoll discontinous density gradient and their different age has been tested by pyruvate kinase (PK) and glucose-6-phosphate dehydrogenase (G6PD) activities.

Each fraction, free of leucocytes, suspended to 10% haematocrit, was filtered through Nuclepore filters 5 micron diameter; erythrocytes lying on filters were observed by Scanning Electron microscopy. Erythrocyte deformability has shown small decrease from middle aged cells to old and youngest ones.

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