A proteomic study on cell cycle progression of endothelium exposed to tumor conditioned medium and the possible role of cyclin D₁/E

Article type: Research Article

Authors: Li, Ailing | Li, Hongwei | Jin, Gang | Xiu, Ruijuan^;

Affiliations: Institute of Microcirculation, Chinese Academy Medical Sciences (CAMS) and Peking Union Medical College (PUMC), Beijing 100005, China | Institute of Mechanics, Chinese Academy of Sciences, Beijing 100080, China

Note: [] Corresponding author. E‐mail: [email protected].

Abstract: This study was designed to comprehensively analyze the differential expression of proteins from human umbilical vein endothelial cells (HUVECs) exposed to tumor conditioned medium (TCM) and to identify the key regulator in the cell cycle progression. The HUVECs were exposed to TCM from breast carcinoma cell line MDA‐MB‐231, then their cell cycle distribution was measured by flow cytometer (FCM). The role of protein in cell cycle progression was detected via two‐dimensional polyacrylamide gel electrophoresis (2‐DE) and western blotting. Following the stimulation of TCM, HUVECs showed a more cells in the S phase than did the negative control group (ECGF‐free medium with 20% FBS), but the HUVECs' level was similar to the positive control group (medium with 25 μg/ml ECGF and 20% FBS). Increased expression of cyclin D1/E and some changes in other related proteins occurred after incubation with TCM. From our results, we can conclude that breast carcinoma cell line MDA‐MB‐231 may secrete soluble pro‐angiogenic factors that induce the HUVEC angiogenic switch, during which the expression of cell cycle regulator cyclin D1/E increases and related proteins play an important role in this process.

Keywords: Tumor conditioned medium, endothelium, cell cycle, 2‐DE, Western blot

Journal: Clinical Hemorheology and Microcirculation, vol. 29, no. 3-4, pp. 383-390, 2003