Affiliations: Section of Endocrinology, Department of Medicine and Department of Clinical Immunology, Aalborg Regional Hospital, Denmark
Note: [*] Request for Reprints: R. Kawahara, M.D., Diabetes Center, Tokyo Women’s Medical College, 10 Kawada-cho, Shinjuku-ku, Tokyo, Japan 162.
Note: [] Accepted by: Editor J.A. Dormandy. Received in Executive Editorial Office 21.5.1985.
Abstract: Filtrability of erythrocytes through polycarbonate sieves with a mean pore diameter of 5 m was studied in fresh blood and stored donor blood before and after rejuvenation with PIGPA and the result was related to simultaneous variations in red cell 2,3-diphosphoglycerate content (2,3-DPG). Following incubation with PIGPA for 2 hours there occurred a significant increase in the content of red cell 2,3-DPG reaching the highest values in fresh blood (14.85 ± 1.05 vs. 4.80 ± 0.65 mmol/l eryth., p < 0.001). The oxygen affinity of hemoglobin simultaneously decreased, and the P50, 7.40 closely correlated with red cell 2,3-DPG content (r = 0.86, p < 0.001). Erythrocyte filtrability of all samples with excess 2,3-DPG content significantly decreased and a negative correlation was present between the concentration of red cell 2,3-DPG and the flow rate (r = −0.67, P < 0.001).
