Studies of erythrocyte membranes by ESR of spin probe

Issue title: Proceedings of the Third European Conference on Clinical Haemorheology. Part I. Baden-Baden, F.R. Germany, 24–27 August 1983

Guest editors: Alfred L. Copley, Holger Schmid-Schönbein and Horst Rieger

Article type: Research Article

Authors: Martini, G.^a | Bobbi, S.^b | Marconi, G.^{b; *} | Morini, R.^c | Albanese, B.^c | Pasquini, G.^c | Manescalchi, P.G.^c | Bartoli, V.^c

Affiliations: [a] Dipartimento di Chimca, University of Florence | [b] Clinica delle Malattie Nervose e Mentali, University of Florence | [c] Patologia Medica IV, University of Florence

Correspondence: [*] Correspondence to Professor Giacomo Martini, Dipartimento di Chimica, Via G. Capponi 9 - 50121 Florence (Italy)

Note: [] Accepted by: Guest Editor M. Hanss and Editor J.A. Dormandy

Abstract: The maleimide-analogue sulfhydryl spin label MAL-6 has been used to study the motional and structural behavior of erythrocyte membrane proteins. Erythrocyte ghosts from insulin-dependent diabetic patients with long-standing complications were mainly examined. The ratios of weakly- and strongly- immobilized -SH groups were valued from the ESR spectra. The results indicate a high degree of resistance of the erythrocyte membrane to rapid bending rather than an increased resistance to translational motion.

Keywords: Spin labels, MAL-6, Erythrocyte Membrane, Viscosity

DOI: 10.3233/CH-1984-42-321

Journal: Clinical Hemorheology and Microcirculation, vol. 4, no. 2-3, pp. 299-309, 1984