Affiliations: Department of Physiology, Faculty of Medicine, Akdeniz University, Antalya, Turkey | Department of Physiology and Biophysics, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA
Note: [] Correspondence to: Oguz K. Başkurt, MD, PhD, Department of Physiology, Akdeniz University, Faculty of Medicine, Kampus, 07070 Antalya, Turkey. Tel.: 90 242 227 4356; Fax: 90 242 2274463; E‐mail: [email protected].
Abstract: Rat red blood cells (RBC) exhibit low aggregation tendency in autologous plasma and in standard aggregating media (e.g., 3% Dextran 70; MW: 70 kD). In experimental studies performed on rats, 3% Dextran 70 was found to be an unsuitable suspending medium to test the “aggregability” of RBC in a standard medium. It has been observed that solutions of higher molecular weight polymers (i.e., dextran, MW: 500 kD; polyethylene glycole, MW: 35 kD; polyvinylpyrrolidone, MW: 360 kD), at low concentrations were strong aggregators for rat RBC. Among these polymer solutions 0.5% Dextran 500 and 0.75% polyvinylpyrrolidone 360 were found to be the most suitable suspending media, to distinguish between the aggregability of RBC from septic and control rats. Therefore, these two polymer solutions are recommended as the standard aggregating media for rat RBC, to test the RBC aggregability.
Keywords: Erythrocyte aggregation, aggregability, standard aggregating media, rat
